FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693161
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAAGA34580.49887399896993634No Hit
ACCACATACTACGCAGACGCCGTGAAGGGCCGTTTCACTACCTCAAGAGA28150.4061105572875565No Hit
GTGTGAGGTGCAGCTGTTGGAATCTGGGGGAGGCTTGGTACAGCCGGGGG24090.3475383063963495No Hit
ATTTATTACTGTGCGAAGAGAGGCTTCGGCTTAGCGAGGCGGGGCGATCT22000.3173865811838808No Hit
GTATGTGGTACTGCCAGTCTCACTAATAGATGAGACCCACTCCAGCCCCT20050.2892545887607641No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18340.2645849954051079No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17920.25852579703705203No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17860.25766019727018685No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17490.2523223320411852No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17100.2466959335565619No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16980.24496473402283164No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16040.23140367100861128No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13670.19721248021743867No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13580.19591408056714096No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13530.1951927474280867No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11760.16965755430556537No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11760.16965755430556537No Hit
GTAGGAGACAGTGTCACCATCACTTGCCGGGCCAGTCAGGGTATTAGTAC11720.16908048779432197No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGGTTTTCAGGTGTT11650.168070621399646No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTC11450.16518528884342887No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11310.1631655560540769No Hit
TCCCTGGACCCTGGCGGACCCAGGTCATGGTATAACTAACAAAGGTGAAT11230.16201142303159005No Hit
CTATTAGTGAGACTGGCAGTACCACATACTACGCAGACGCCGTGAAGGGC11220.16186715640377922No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10770.15537515815229075No Hit
ATACTACGCAGACGCCGTGAAGGGCCGTTTCACTACCTCAAGAGACAATT10720.15465382501323646No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT10370.14960449303985654No Hit
GGGTATTAGTACCGGGTTGGCCTGGTATCAGCAGAAATCAGGGAAAGCCC10240.1477290268783154No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10230.14758476025050457No Hit
GTCCAGGGAAGGGGCTGGAGTGGGTCTCATCTATTAGTGAGACTGGCAGT9980.1439780945552332No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9770.14094849537120524No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG9440.13618769665344704No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT9240.13330236409722993No Hit
GTATTAGTACCGGGTTGGCCTGGTATCAGCAGAAATCAGGGAAAGCCCCT9240.13330236409722993No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9150.13200396444693224No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8820.12724316572917402No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8690.1253676995676329No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGTTAGTTATACCATGACCTGGG8680.12522343293982205No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTGTTAGTTATACC8680.12522343293982205No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8610.12421356654514608No Hit
GCATGATAGTTACCCACTCACTTTTGGCGGAGGGACCAGGGTGGACATCA8580.1237807666617135No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGTGTCACCATCACTTGCCG8480.12233810038360497No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8450.1219053005001724No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA8440.12176103387236153No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG8350.12046263422206384No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8340.12031836759425299No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC8330.12017410096644215No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTCCTTGT8270.119308501199577No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8220.11858716806052273No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC8130.11728876841022504No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC8100.11685596852679249No Hit
CACTAATAGATGAGACCCACTCCAGCCCCTTCCCTGGACCCTGGCGGACC8090.11671170189898163No Hit
CTATAAGGCATCTACTTTAGAAAATGGGGTCCCTTCAAGGTTCGGCGGCA8070.11642316864335991No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT8010.11555756887649479No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7990.11526903562087308No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7860.11339356945933197No Hit
GTTATACCATGACCTGGGTCCGCCAGGGTCCAGGGAAGGGGCTGGAGTGG7820.11281650294808854No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC7710.11122957004216913No Hit
TAGTTATACCATGACCTGGGTCCGCCAGGGTCCAGGGAAGGGGCTGGAGT7660.11050823690311486No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG7640.11021970364749314No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT7550.10892130399719545No Hit
TTATTACTGTGCGAAGAGAGGCTTCGGCTTAGCGAGGCGGGGCGATCTGA7480.10791143760251946No Hit
CAGTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAA7460.10762290434689777No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7450.10747863771908692No Hit
CTATCATGCTGTTGACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT7440.10733437109127605No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT7310.10545890492973495No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7260.10473757179068066No Hit
GCGTAGTATGTGGTACTGCCAGTCTCACTAATAGATGAGACCCACTCCAG7250.1045933051628698No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC7230.10430477190724811No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG7100.10242930574570698No Hit
GTCTGCATCTGTAGGAGACAGTGTCACCATCACTTGCCGGGCCAGTCAGG7090.10228503911789613No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA7090.10228503911789613No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC7070.10199650586227443No Hit
GTATCAACGCAGAGTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGG7030.101419439351031No Hit
GGGTGGACATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG6940.1001210397007333No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)