FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693161
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAAGA29370.4237110858804809No Hit
ACCACATACTACGCAGACGCCGTGAAGGGCCGTTTCACTACCTCAAGAGA21400.30873058351522953No Hit
GTGTGAGGTGCAGCTGTTGGAATCTGGGGGAGGCTTGGTACAGCCGGGGG20130.29040872178325094No Hit
ATTTATTACTGTGCGAAGAGAGGCTTCGGCTTAGCGAGGCGGGGCGATCT19200.2769919253968414No Hit
GTATGTGGTACTGCCAGTCTCACTAATAGATGAGACCCACTCCAGCCCCT16370.23616446972636948No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15740.22707567217428562No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15460.22303620659558168No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15180.21899674101687774No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15080.21755407473876923No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14070.20298314532987285No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13350.1925959481274913No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12970.18711381627067883No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11340.1635983559375095No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11200.1615786231481575No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10550.15220129234045193No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10520.15176849245701937No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9380.1353220968865819No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9360.1350335636309602No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGGTTTTCAGGTGTT9350.13488929700314933No Hit
CTATTAGTGAGACTGGCAGTACCACATACTACGCAGACGCCGTGAAGGGC9110.13142689793568882No Hit
TCCCTGGACCCTGGCGGACCCAGGTCATGGTATAACTAACAAAGGTGAAT8980.1295514317741477No Hit
GTAGGAGACAGTGTCACCATCACTTGCCGGGCCAGTCAGGGTATTAGTAC8860.12782023224041744No Hit
GTCCAGGGAAGGGGCTGGAGTGGGTCTCATCTATTAGTGAGACTGGCAGT8850.1276759656126066No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8790.12681036584574146No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8770.12652183259011976No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTC8730.12594476607887634No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8730.12594476607887634No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8690.1253676995676329No Hit
ATACTACGCAGACGCCGTGAAGGGCCGTTTCACTACCTCAAGAGACAATT8560.1234922334060918No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8420.12147250061673982No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT7860.11339356945933197No Hit
GTATTAGTACCGGGTTGGCCTGGTATCAGCAGAAATCAGGGAAAGCCCCT7820.11281650294808854No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG7740.1116623699256017No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG7710.11122957004216913No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTCCTTGT7580.10935410388062802No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7520.1084885041137629No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC7520.1084885041137629No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7470.1077671709747086No Hit
GGGTATTAGTACCGGGTTGGCCTGGTATCAGCAGAAATCAGGGAAAGCCC7400.10675730458003263No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGTTAGTTATACCATGACCTGGG7350.10603597144097836No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC7080.10214077249008527No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC7060.10185223923446357No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7040.10156370597884186No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9150.070.8891141
ATCAACG11650.054.3728333
TCAACGC11650.054.3728334
TATCAAC12600.050.2732932
CAACGCA12500.050.0996255
AACGCAG13000.048.1727186
CGCAGAG13250.046.7205358
GGCAGCG5500.045.8068667
CAGCGCT5700.042.936769
GCAGCGC5950.042.342488
GCAGAGT15000.041.2698069
TATTAGG2005.456968E-1239.590222
CAGGGCA7700.032.719194
TCTATAC1551.5484438E-632.50813
TATGTAC10250.032.304222
TACACGG9850.032.154496
GTATTAC1352.2300128E-532.0313761
GAGCGTC900.003757036531.9920989
TACATGG19500.031.7460062
ACATGGG19300.031.7020133
Produced by FastQC (version 0.11.5)