FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579021
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19360.334357475808304No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16230.28030071448185817No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCATCAGGGAGA15050.2599214881670958No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11720.20241062068560553No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10240.1768502351382765No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG10080.17408695021424095No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC9420.1626883999025942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9240.1595797043630542No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT9210.15906158843979754No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT8040.13885506743278742No Hit
GTGTATTACTGTGCGAGAGACCGGGGATATCGTGGTCACTTGGACCACTG8030.13868236212503518No Hit
GTCTCTGGTGACTCCATCACCAGTGATAACTGGTGGAGTTGGGTCCGCCA7440.128492748967654No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7070.12210265258082177No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG7060.12192994727306954No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT6840.11813043050252063No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA6790.11726690396375952No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC6340.10949516511490948No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC5880.10155072095830722No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT5800.10016907849628943No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)