FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579021
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21160.3654444312037042No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCATCAGGGAGA15050.2599214881670958No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14890.25715820324306027No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG12050.2081098958414289No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT9550.16493356890337313No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC9190.15871617782429306No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT9030.15595289290025752No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG8310.14351811074209744No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8090.13971859397154854No Hit
GTGTATTACTGTGCGAGAGACCGGGGATATCGTGGTCACTTGGACCACTG8030.13868236212503518No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7630.13177414981494626No Hit
GTCTCTGGTGACTCCATCACCAGTGATAACTGGTGGAGTTGGGTCCGCCA7410.12797463304439735No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC7020.12123912604206065No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG6740.11640337742499841No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC6350.1096678704226617No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC6260.10811352265289169No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT6230.10759540672963502No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA5920.10224154218931611No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG1800.047.989899
ATCAACG7300.047.369323
TCAACGC7350.047.047084
GTCATAG7500.046.1260761
CAGAGCG8050.044.7110829
ATAGGTG7900.044.683564
CAACGCA7850.044.050455
TATCAAC8550.041.2901342
TAGGTGG8550.040.4439855
AACGCAG8450.040.0423666
GTATCAA8950.039.458411
CATAGGT8600.039.371163
TCATAGG8650.038.314052
ACGCAGA8850.037.4190837
GTGCTAG2750.036.6911931
CGCAGAG9200.035.995538
GGTATCA3900.035.1119961
AGGTGGT10500.032.2245676
TGCACAC12000.030.0299971
CACACAC13350.028.6004833
Produced by FastQC (version 0.11.5)