FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584957
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC53510.33761168284060705No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCAGTA44560.28114327391847227No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGTTCTG42510.2682091690815587No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA39720.25060616786449097No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35820.225999822077192No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33500.21136220099346545No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGATACTGC33490.21129910779913905No Hit
CCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG29230.1844214070160894No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29120.1837273818784989No Hit
GGTATCAGCAGATGCCAGGCCAGGCCCCTGTCCTTGTCATCTATTATGAT28160.17767043522316378No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA28060.1770395032798997No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCACCTGGTGGAGTCTGGGGGAGGCG28040.1769133168912469No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27420.17300153884300962No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA27260.1719920477337871No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC26580.16770171051959137No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25600.16151857747560344No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT25140.1586162905365887No Hit
CTATAAAGACTCCCTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCA24260.1530640894358648No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC24070.15186531874366307No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA23850.1504772684684821No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23440.1478904475010994No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22800.1438524830642093No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA22590.14252752598335477No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22170.13987761182164563No Hit
GAAATACACAGACGTGTCGTCAGGTCTCAGGGAGTTCATTTGGAGATAAA22010.1388681207124231No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21790.13748007043724214No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCAGTAG21350.13470396988688021No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA20390.1286470232315451No Hit
GTGTGGGATAGCGCTACTGATCATTGGGTGTTCGGCGGAGGGACCAAGCT20120.12694350698473209No Hit
GTCCTTGTCATCTATTATGATAACGACCGGCCCTCAGGGATCACTGAGCG20090.12675422740175285No Hit
CTCGTGTACAGTCTCTGGCTTCTCCTTCAGTGCCCATGCCATGCACTGGG19980.12606020226416237No Hit
GGTTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGC19850.12523999073791905No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19520.12315791532514762No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19430.12259007657620996No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19420.12252698338188356No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19040.12012944199748005No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA19020.12000325560882724No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC18900.11924613727691034No Hit
CCTCTCTCACTGCACAGGTTCTGTGACCTCCTATGTGCTGACTCAGCCAC18800.11861520533364628No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18550.1170378754754861No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18540.11697478228115966No Hit
GTCTCTGGCTTCTCCTTCAGTGCCCATGCCATGCACTGGGTCCGCCAGGC18230.11501889325704104No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18220.11495580006271464No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17740.11192732673504709No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17590.11098092882015095No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT17580.11091783562582455No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA17410.10984525132227561No Hit
GTGTATTTCTGTGCGACGGGATATAGTGGCTACCCGACACCTGAATACCT17110.10795245549248339No Hit
CTATTATGATAACGACCGGCCCTCAGGGATCACTGAGCGATTCTCTGGCT17070.10770008271517777No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16940.10687987118893447No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT16830.10618584605134399No Hit
CTATATCCCGTCGCACAGAAATACACAGACGTGTCGTCAGGTCTCAGGGA16620.10486088897048941No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16590.1046716093875102No Hit
GTCTTTATAGAATATCTTAGTTCCATCAAACGAAATGACTCCCATCCACT16440.1037252114726141No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT16420.10359902508396127No Hit
CCCTGAGACCTGACGACACGTCTGTGTATTTCTGTGCGACGGGATATAGT16310.1029049999463708No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT16140.10183241564282186No Hit
GAACTAAGATATTCTATAAAGACTCCCTGAAGGGCCGATTCACCATCTCC16100.10158004286551622No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16070.10139076328253702No Hit
GTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACGGCCAG15970.10075983133927292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26200.075.853361
ATCAACG31950.061.751393
TCAACGC32150.061.143284
TATCAAC33900.058.1993142
CAACGCA34650.056.314395
AACGCAG36000.054.0042956
CGCAGAG40500.048.35948
GCAGAGT45500.043.039759
GAATACG1903.6379788E-1241.6822669
ACGCAGA47950.040.5454567
GTACATG41650.038.207121
TACATGG42250.037.6645362
TATTGAG19000.036.0017245
ACATGGG45100.034.9650843
CAGCGCT10050.032.23739
TTATTGA21050.032.1545754
CATGGGA25450.030.8393884
TAGCGTA1000.0062819828.8022887
GGCAGCG11450.028.2991947
TACACGG17350.027.8062486
Produced by FastQC (version 0.11.5)