FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508339
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14500.2852427218844118No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.23350559370813573No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11100.21835822158048074No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG6010.11822819024312516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5880.11567084170209249No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.10583488577504381No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA450.00990788847.994628
AGTACGG1252.8672548E-740.319455
ACCAGCG1157.488532E-637.561018
GTACGGA1406.9693124E-735.9995086
ATAAGAC2051.2134478E-831.6155453
GCCCTAA1204.208801E-430.017311
AACGTGT1000.00628389928.7967747
CGGATGC1856.1351384E-627.240199
GTATCAA42300.026.7388211
ATCAACG41300.026.6780093
CAACGCA41350.026.6405075
TCAACGC41400.026.613574
AACGCAG41900.026.4626466
CGCAGAG42300.026.2098278
TATCAAC42950.025.9884622
GGAGCAT2259.667856E-725.5971328
ACGCAGA43900.025.2545727
TAGTACG3451.1641532E-1025.0480654
GTAGTTC2901.2758392E-824.8321323
ACGGATG2051.3608114E-524.5826118
Produced by FastQC (version 0.11.5)