FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508339
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13770.2708822262309207No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11300.22229260395130024No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT5530.10878567255315841No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5510.10839223431607647No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACC950.004882586730.3224014
AGTACGG1504.600003E-528.806285
TACACGC1255.356345E-428.8062785
GTACGGA1607.149641E-527.0005726
GTACTAG2705.9553713E-926.6751081
TTACACG1358.399662E-426.6724834
ACGCTTG1400.001040403325.71238
CGCTTGC1400.001040403325.71239
CATGGGG10950.025.6494274
GACCGCG1701.0797657E-425.4123027
TGGACCG1751.3129396E-424.6910975
ACTACGT1801.589492E-424.0052363
GACTCGG2701.7589628E-724.000517
TAGTACG4353.6379788E-1223.1774674
GGACTCG2852.9639887E-722.7373246
CGGATGC1902.297421E-422.7350859
TATACAG5001.8189894E-1221.604715
GTACATG34650.021.4093721
GCCCATA3106.639875E-720.9098431
TACATGG35000.020.5759142
Produced by FastQC (version 0.11.5)