FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974157
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGGAACA57770.5930255595350648No Hit
GTCTACTACTGTGCGAGATTCGGTTTCCCCAATGACGACAACTGGTTCGA48310.4959159560522585No Hit
TACTTATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGA32380.3323899535701124No Hit
CTCTTAGACTCTCCTGTGTAGGCTCTGGATTCACCTTCAGTGACTATAGC23220.23835993582143328No Hit
CCTCATATATGATGCATCGAACAGGGCCACTGGCATCCCAGCCAGGTTCA22410.23004505433929026No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA20390.20930917706283486No Hit
GTATAAGTAACTTCTACTACTACGACTGATGGTTGAGACCCACTCCAGCC20300.20838530134259675No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT19320.19832532127778169No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT18920.19421920696561232No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT18630.1912422740892895No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT18410.18898391121759633No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15220.15623764957804542No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT14220.14597236379762193No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG13410.13765748231547892No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG12340.1266736265304258No Hit
TATATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAA12320.12646832081481735No Hit
CTCCTGTGTAGGCTCTGGATTCACCTTCAGTGACTATAGCATGAACTGGG12220.12544179223677498No Hit
ATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACG12170.1249285279477538No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG12040.12359404079629874No Hit
CTATAGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGG11620.11928262076852088No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11390.11692160503902348No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11340.11640834075000231No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC11200.11497120074074302No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCT10900.11189161500661597No Hit
CTCCAGAGACAACGCCTGGAACTCACTGTTCCTGCAAATGAACAGCCTGA10640.10922264070370588No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA10630.10911998784590164No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA10560.10840141784127198No Hit
GTGTAGGCTCTGGATTCACCTTCAGTGACTATAGCATGAACTGGGTCCGC10550.10829876498346774No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGATTCCAAGGCCTT10460.10737488926322965No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG10380.10655366640079578No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA10120.10388469209788564No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA9810.10070245350595437No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT9810.10070245350595437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6550.072.709751
GTATCAA16850.065.521151
TCAACGC17300.062.421024
CAACGCA17800.060.6676255
ATCAACG17800.060.6676253
AACGCAG18900.056.7557956
TATCAAC19350.055.8480762
GAATACG2600.047.0718579
CAGCCGT22050.045.382879
CGCAGAG23950.044.7884988
TAGACAG23100.043.0083585
AGTAGAC22850.042.8487783
GTGCTAG3150.041.2336651
GTAGACA24850.040.269314
GCAGAGT27000.039.7290579
GTACACG9650.038.0477185
TACACGG9650.038.0477186
AGACAGC27200.037.8488666
GTATGTA11250.034.6362841
ACATGGG22750.034.4929923
Produced by FastQC (version 0.11.5)