FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974157
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGGAACA45780.4699447830277871No Hit
GTCTACTACTGTGCGAGATTCGGTTTCCCCAATGACGACAACTGGTTCGA40510.41584672696495534No Hit
TACTTATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGA25000.2566321445105871No Hit
CTCTTAGACTCTCCTGTGTAGGCTCTGGATTCACCTTCAGTGACTATAGC19560.20078898986508337No Hit
CCTCATATATGATGCATCGAACAGGGCCACTGGCATCCCAGCCAGGTTCA18790.19288471981415725No Hit
GTATAAGTAACTTCTACTACTACGACTGATGGTTGAGACCCACTCCAGCC17550.18015576544643216No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA16620.1706090496706383No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT16040.1646551839179927No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT15010.1540819395641565No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT14970.15367132813293957No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14640.1502837838253998No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13510.13868401089352128No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT11730.12041180220436748No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG11670.11979588505754207No Hit
TATATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAA11540.11846139790608701No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG10650.1093252935615101No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA10470.10747754212103387No Hit
CTCCTGTGTAGGCTCTGGATTCACCTTCAGTGACTATAGCATGAACTGGG10430.10706693068981694No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10240.10511652639153647No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10030.10296081637764755No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCT10030.10296081637764755No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC11100.062.2564434
ATCAACG11600.060.8140753
CAACGCA12150.056.876255
AACGCAG12750.055.3288846
GTATCAA13450.055.1904561
TATCAAC13850.050.9345362
GGTATCA5400.050.7152861
CGCAGAG15250.043.8984458
GTACATG22900.043.4302831
TACATGG23400.041.5292362
ACATGGG23350.041.00163
AGTAGAC21150.040.5016443
CAGCCGT19600.040.0319259
TAGACAG21700.039.8068285
GTAGACA23250.037.7722554
GAATACG3200.035.9920049
GTGCTAG4300.035.196531
GCAGAGT19500.034.699989
ATTACGG1051.9331314E-434.2781038
AGACAGC26050.033.712286
Produced by FastQC (version 0.11.5)