FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671415
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAAGCAGAGGCCAGGCCAGGCCCCTGCGCTGGTCGTCTATGATGAT67080.4013365920492517No Hit
GTACTAGTGATCATCCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA37190.2225060801775741No Hit
CACTAGTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCT36810.22023255744384249No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC32910.19689903465028133No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGATGATCACTA30550.18277926188289564No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG29720.17781340959606082No Hit
GCTTATACCAGTGCACACTTTTACCTCCAATGTTGTCTCCCTCACAGGTA27850.16662528456427636No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC27720.16584750047115768No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24960.1493345458787913No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA23320.13952250039637074No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT22620.13533443220265462No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22130.13240278446705336No Hit
GTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC20830.12462494353586633No Hit
CCTATGTGCTGACTCAGCCACCCACGGTGTCACTGGCCCCAGGAAAGACG20630.123428352623376No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGG20310.12151380716339148No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA20180.1207360230702728No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC19610.11732573896967538No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA19100.1142744321428251No Hit
GTCATGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT19080.11415477305157606No Hit
GTAATAGACGGCCGTGTCGTCATACTTCAGGCTGCTCAGTTCCATGTAGG18830.11265903441096317No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18290.10942823894723931No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17960.1074538639416303No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17290.10344528438478774No Hit
GTGTGGGATAGTACTAGTGATCATCCGGTGTTCGGCGGAGGGACCAAGCT16970.10153073892480323No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33200.078.288291
GGTATCA13550.071.202171
TCAACGC41250.062.6667484
ATCAACG41350.062.51523
CAACGCA43450.059.162315
TATCAAC44250.058.418162
AACGCAG45450.056.5589076
ACGCAGA53050.048.0490237
CGCAGAG53100.048.003788
GCAGAGT61950.041.1399429
TAGACGG10600.032.6063425
GTACATG55350.032.2598081
TATACGG950.004889081730.3181782
ACATGGG55500.030.0996673
CATGGGA28250.027.5278324
TACATGG63150.027.2515532
CAGAGTA58250.025.9552429
GGTATAA42550.025.0432031
ATGGGAG22950.024.1587665
TTATTGA23850.023.5490254
Produced by FastQC (version 0.11.5)