FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700989
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCGTCTCCAGAGA10800.15406803815751743No Hit
ATAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA10660.1520708598851052No Hit
GTATATGTAACTTCCACTACTACTCATGGTTGAGACCCACTCCGGCGCCT10180.14522339152254884No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT9980.14237027970481705No Hit
GTCTATTATTGTACGAGATTCGGTTGGCCCAATGACGACAATTGGTTCGA9610.13709202284201322No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA7510.10713434875582925No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG7220.10299733662011816No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC7130.10171343630213883No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC7200.049.00694
ATCAACG7400.047.6857873
CAACGCA7650.047.0654535
GTATCAA8500.044.0564041
GGTATCA4550.042.7343371
AACGCAG8450.042.6034666
TATCAAC9250.040.4842642
CGCAGAG9650.037.3056268
GTACATG16150.034.3354571
TACATGG15850.033.167922
GCAGAGT12150.029.6295729
TAGGACA2701.8553692E-1029.3374634
ACATGGG19150.027.8283563
CCCTATA2808.792995E-925.7197422
CCTATAC3659.094947E-1225.6492753
CTATACC3659.094947E-1225.6474444
CAGAGTA13450.024.6244879
GGACTCG5350.024.224256
GTAGGAC1801.5903958E-424.0050933
GATAGAC1550.001882237823.225767
Produced by FastQC (version 0.11.5)