FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376454
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAAGAGGTGTCCAGTGTCAGGTGCGGCTGGTGGAGTCTGGGGGAGGCG42530.17896412049212818No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC41180.17328338777018196No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT37060.15594663309283496No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC34900.146857460737721No Hit
CTCCAGAGACGATTCCAAGAACACCGTCTATCTCCAAATGAACAGCCTGA34680.14593171170155197No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT33460.14079801250097834No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC31720.1334761792149143No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA31460.13238211217216914No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT31220.131372204132712No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCTTTGACAC31070.13074101160805132No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30700.1291840700472216No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA30670.12905783154228948No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA29990.12619642543049434No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCTTTGACA29700.12497611988281701No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC29320.12337709882034324No Hit
GTACAATGGTGGCCCTCCACGCCATTCGCACAATAATAAAAAGCCGTGTC28520.12001073868881956No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA28230.11879043314114222No Hit
CTGTAGCAGGTATTCCCTGTACAATGGTGGCCCTCCACGCCATTCGCACA28070.1181171611148375No Hit
TCCCTGTACAATGGTGGCCCTCCACGCCATTCGCACAATAATAAAAAGCC26970.11348841593399242No Hit
TCTCTGGAGAGGGTGAATCGGCCCTTCACGGAGTCTGCGTAATATTCAGT26930.11332009792741622No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26520.11159483836001033No Hit
GTACAGGGAATACCTGCTACAGTGGTCCATTCTACTACTACGGTATGCAT26290.11062700982219728No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC25390.10683985467423313No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT24750.10414676656901416No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC24450.1028843815196928No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG24070.10128536045721903No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37200.080.172881
TCAACGC44250.066.5533454
ATCAACG45500.064.883213
GGTATCA14550.064.36511
CAACGCA46550.063.4196785
TATCAAC46950.062.8793562
AACGCAG48150.061.1601526
CGCAGAG56750.051.8896528
GCAGAGT67450.043.551349
GTACATG63900.034.2722661
TACATGG64750.033.361172
ACATGGG63500.033.224143
TACACGG18450.031.6104156
GTACACG18250.031.5636315
ACGCAGA101900.028.8983087
GTATGTA24050.028.755851
ACACGGG21650.026.9370757
TATGTAC21800.026.7539772
ACGGGGT22450.026.618599
CATGGGG41700.025.5555674
Produced by FastQC (version 0.11.5)