FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376454
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAAGAGGTGTCCAGTGTCAGGTGCGGCTGGTGGAGTCTGGGGGAGGCG37750.15885011870627413No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC34730.14614210920977222No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT34450.14496388316373893No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGACGCAGCCACCCTCAGCGTCT34290.14429061113743416No Hit
GTCCTGGGCCCAGTCTGTCCTGACGCAGCCACCCTCAGCGTCTGGGACCC32200.13549599529382853No Hit
CTCCAGAGACGATTCCAAGAACACCGTCTATCTCCAAATGAACAGCCTGA30490.12830040051269664No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30450.12813208250612046No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT30100.12665929994857883No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT30020.12632266393542646No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC29660.12480780187624083No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC29260.12312462181047898No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACGCAGCCAC26150.11003789679918062No Hit
GTACAATGGTGGCCCTCCACGCCATTCGCACAATAATAAAAAGCCGTGTC25750.10835471673341877No Hit
CTGTAGCAGGTATTCCCTGTACAATGGTGGCCCTCCACGCCATTCGCACA25560.10755520620218191No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA25010.10524083361175937No Hit
TCCCTGTACAATGGTGGCCCTCCACGCCATTCGCACAATAATAAAAAGCC24940.10494627710025105No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24510.10313685852955706No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC24050.10120120145393095No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA24020.10107496294899879No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37100.079.385531
GGTATCA15050.065.550511
TCAACGC45000.065.279324
ATCAACG45500.064.245493
CAACGCA49150.059.767445
TATCAAC49750.059.6255072
AACGCAG50950.057.797246
CGCAGAG58800.050.0811128
GCAGAGT67600.043.2394269
GTACATG63050.032.207421
TACATGG63900.030.8729172
ACATGGG64250.030.1444363
ACGCAGA100250.029.5178997
TACACGG18800.027.574186
GTACACG18400.027.391025
GTATGTA23950.026.7594051
CAGAGTA63500.025.2831829
TATGTAC22400.024.1068922
ACACGGG21700.023.5573587
CAGCGCT13950.023.22419
Produced by FastQC (version 0.11.5)