FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920608
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG74360.3871690631300088No Hit
GATTATTACTGTGCAGTGTGGGATGTCAGCCGGGGTGAGTGGGTGTTCGG47890.24934812309435345No Hit
GTAATAGACGGCCGTGTCCTCAGACCTGAGGCTGCTCAGTTCCATGTAGG45410.23643554541061995No Hit
GTGTGGGATGTCAGCCGGGGTGAGTGGGTGTTCGGCGGAGGGACCAAGCT41620.21670221096652728No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGTGTCTGGGACCC41130.21415093553707995No Hit
ATCCCACACTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC39970.20811118145920457No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA39730.2068615771672304No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGTGTCT39340.2048309701927723No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39180.20399790066478948No Hit
GTCTATTACTGTGCGAGCCAGTTATATTGTAATAGTAGTGACTGTGCTCC33030.17197679068295038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28680.14932771289091787No Hit
GTCTGGGGCAGAGGTGAAGAAGCCTGGGTCCTCGGTGACGGTCTCCTGCA28550.14865084389943184No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC28240.14703677168896515No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27850.14500616471450706No Hit
GCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAG25880.1347489961512188No Hit
GTGCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGCAGAGGGTCAC25180.131104316966294No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25070.13053158166580583No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA24460.12735550409037139No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC24020.12506456288841866No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23850.12417942651493694No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT23600.1228777553774638No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA22730.11834793981905731No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGAAGCT22500.11715040237258202No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC22270.11595286492610674No Hit
TTACTGTGCAGTGTGGGATGTCAGCCGGGGTGAGTGGGTGTTCGGCGGAG22170.11543219647111747No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTT21700.11298505473266798No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21630.1126205868141755No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21570.11230818574118197No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21140.11006931138472817No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20210.10522709475332812No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC20130.10481055998933671No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19920.10371715623385927No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19790.10304028724237325No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC19270.10033281127642914No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19240.10017661073993235No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)