FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920608
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG57880.30136290174777985No Hit
GATTATTACTGTGCAGTGTGGGATGTCAGCCGGGGTGAGTGGGTGTTCGG41140.21420300238257886No Hit
GTAATAGACGGCCGTGTCCTCAGACCTGAGGCTGCTCAGTTCCATGTAGG39110.20363343274629703No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGTGTCTGGGACCC38190.19884328296039588No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGTGTCT38010.1979060797414152No Hit
GTGTGGGATGTCAGCCGGGGTGAGTGGGTGTTCGGCGGAGGGACCAAGCT37480.19514653692997216No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37390.19467793532048186No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA35900.18691997534114196No Hit
ATCCCACACTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC31980.16650977190556324No Hit
GTCTATTACTGTGCGAGCCAGTTATATTGTAATAGTAGTGACTGTGCTCC29050.1512541861743781No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26980.14047634915610058No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26160.13620686782518868No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC25610.1333431913227478No Hit
GTCTGGGGCAGAGGTGAAGAAGCCTGGGTCCTCGGTGACGGTCTCCTGCA25290.1316770522667822No Hit
GTGCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGCAGAGGGTCAC24460.12735550409037139No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23960.12475216181542512No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT22790.11866034089205084No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA22200.11558839700761425No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGAAGCT22040.11475532747963145No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21670.11282885419617121No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21040.10954864292973893No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20540.10694530065479264No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA20410.10626843166330663No Hit
GCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAG20080.10455022576184207No Hit
TTACTGTGCAGTGTGGGATGTCAGCCGGGGTGAGTGGGTGTTCGGCGGAG19620.10215515086889151No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC19540.1017386161049001No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)