FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330804
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16130.48759990810268317No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14910.45072006384445173No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10600.32043143371906024No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG9980.30168921778454916No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT8880.2684368991910618No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG8200.24788092042417864No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC7750.23427769918138835No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT7540.22792952926808624No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7000.21160566377673787No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG6430.19437491686920352No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC6360.19225886023143615No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC6130.18530610270734332No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.1843992212911573No Hit
CCCTGAGGAGACGGCGACCAGGGTTCCCTGGCCCCAGTAGTTGGATCCCC5940.17956252040483187No Hit
GATTAAGACTGATGGGAGTGTAACAAGCTACGCGGACTCCGTGAAGGGCC5080.15356525314083264No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT5050.1526583717246466No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAGTGTTCCTG5030.15205378411385595No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC4860.14691478942213515No Hit
GTCTTAATCTGTGAGACCCATTCCGGCCCCTTCCCTGGAGGTTGGCGGAT4840.14631020181134446No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC4700.14207808853580972No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG4700.14207808853580972No Hit
GTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCG4650.14056661950883304No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC4510.13633450623329826No Hit
GAAGTATACCGCCGTGTCCTCGGCTCTCAGTCTGTTCATTTGCAGATACA4360.13180009915236818No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG4320.1305909239307868No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA4320.1305909239307868No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC4220.1275679858768334No Hit
GTGTGAGGTGCAGCTGGTGGAGTCCGGGGGAGGCTTAGTTCAGCCTGGGG4190.12666110446064738No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAAAGAAGTT4070.12303357879590332No Hit
GACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCA4030.12182440357432196No Hit
CTCCAGAGACAACGCCAAGAACACGCTGTATCTGCAAATGAACAGACTGA3940.1191037593257639No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC3930.11880146552036856No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCA3900.11789458410418255No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA3800.11487164605022913No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT3790.1145693522448338No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCCGGGGGAGGCT3750.11336017702325243No Hit
CACGTGCACCGTGACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCA3600.10882576994232233No Hit
CTACTGGATGCACTGGATCCGCCAACCTCCAGGGAAGGGGCCGGAATGGG3590.10852347613692699No Hit
TGCAGATACAGCGTGTTCTTGGCGTTGTCTCTGGAGATGGTGAATCGGCC3580.10822118233153166No Hit
GTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGC3530.10670971330455495No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCATCTTCGGTGACTACTGG3490.10550053808297359No Hit
GTATACTTCTGCGCACGAGATCTCAGGGGATCCAACTACTGGGGCCAGGG3410.10308218763981089No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT3390.10247760002902022No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTCGGTGACTACTGGATGCACTGGA3380.10217530622362488No Hit
GTATACCGCCGTGTCCTCGGCTCTCAGTCTGTTCATTTGCAGATACAGCG3380.10217530622362488No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC3330.10066383719664818No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT3320.10036154339125283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG1350.058.6995121
ATCAACG6800.058.2502753
TCAACGC6950.056.9930764
CAACGCA7200.055.0058335
AACGCAG7500.053.76576
ACGCAGA8350.048.2779467
CGCAGAG8350.048.2779468
GAATACG1352.0372681E-1047.9905789
GCAGAGT9300.043.346339
TAGGTGG5400.042.6711925
ATAGGTG5550.041.5241934
TACTAAT700.001090790341.1534424
GTCATAG5650.040.8015941
GTATCAA10350.038.9783331
GTATAGA750.001528998338.42151
ATGCAAC1502.7399437E-838.42151
CTAGAGA1701.9444997E-938.1274534
CAGAGCG4750.037.88739
AGAATAC1752.593879E-937.0213058
GGTATCA6450.035.740931
Produced by FastQC (version 0.11.5)