FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330804
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18150.5486632567925418No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14640.4425581310987775No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG12350.3733328496632447No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT8780.26541396113710836No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT8550.25846120361301556No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC8490.25664744078064355No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8170.24697403900799264No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG7060.2134194266091099No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC6830.2064666690850171No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC6430.19437491686920352No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA5650.170796000048367No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG5210.15749507261097206No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT5120.154774428362414No Hit
GATTAAGACTGATGGGAGTGTAACAAGCTACGCGGACTCCGTGAAGGGCC5030.15205378411385595No Hit
CCCTGAGGAGACGGCGACCAGGGTTCCCTGGCCCCAGTAGTTGGATCCCC4960.14993772747608855No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC4900.14812396464371652No Hit
GAAGTATACCGCCGTGTCCTCGGCTCTCAGTCTGTTCATTTGCAGATACA4900.14812396464371652No Hit
GTCTTAATCTGTGAGACCCATTCCGGCCCCTTCCCTGGAGGTTGGCGGAT4870.1472170832275305No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC4760.14389185136818175No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAGTGTTCCTG4640.1402643257034377No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA4430.13391615579013555No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC4400.13300927437394952No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG4330.13089321773618215No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG4260.12877716109841478No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT4210.12726569207143806No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG4180.12635881065525204No Hit
CTCCAGAGACAACGCCAAGAACACGCTGTATCTGCAAATGAACAGACTGA4090.123638166406694No Hit
GTGTGAGGTGCAGCTGGTGGAGTCCGGGGGAGGCTTAGTTCAGCCTGGGG4080.12333587260129866No Hit
GTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGC4070.12303357879590332No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT3940.1191037593257639No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC3930.11880146552036856No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC3900.11789458410418255No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT3900.11789458410418255No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCCGGGGGAGGCT3870.11698770268799652No Hit
GTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCG3830.11577852746641516No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTCGGTGACTACTGGATGCACTGGA3780.11426705843943846No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAAAGAAGTT3640.11003494516390369No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCA3570.10791888852613632No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCATCTTCGGTGACTACTGG3560.10761659472074098No Hit
GACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCA3520.10640741949915962No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCA3470.10489595047218292No Hit
GTATACCGCCGTGTCCTCGGCTCTCAGTCTGTTCATTTGCAGATACAGCG3430.10368677525060158No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC3370.10187301241822953No Hit
CTACTGGATGCACTGGATCCGCCAACCTCCAGGGAAGGGGCCGGAATGGG3350.10126842480743885No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)