FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769971
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTTCAGATCTCAGGCTGCTCAGTTTCATGTAGG22570.29312792299969737No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC19480.2529965414281837No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT18510.2403986643652813No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCCACTA17220.22364478662183382No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCCACTAC17130.2224759114304305No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT16710.21702116053721504No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC16470.21390416002680623No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16420.21325478492047104No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT16410.213124909899204No Hit
GTGTATTACTGTGCGTGCGGTGACTACGGTGACTATCTTGACTACTGGGG16090.20896890921865888No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA14610.18974740607113774No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC14010.18195490479511567No Hit
GTAATATTCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG13910.1806561545824453No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCT13590.1765001539019002No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12710.16507115203040115No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAACGTTTCCTGCA12390.16091515134985604No Hit
GAATATTACTGCTACTCATATGCAGGTAGTGGCACTTATGTCTTCGGAAC11870.15416165024397022No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC11750.1526031499887658No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11450.14870689935075476No Hit
GCATATGAGTAGCAGTAATATTCAGCCTCGTCCTCAGCCTGGAGCCCAGA11370.14766789918061848No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11300.14675877403174925No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC11260.1462392739466811No Hit
GTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA11200.1454600238190789No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA11110.1442911486276756No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10920.14182352322360195No Hit
CAGTAATACACAGCCGTGTCTTCAGATCTCAGGCTGCTCAGTTTCATGTA10670.13857664769192607No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT10560.13714802245798868No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10240.13299202177744357No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG9770.12688789577789292No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9770.12688789577789292No Hit
GTATTACTGTGCGTGCGGTGACTACGGTGACTATCTTGACTACTGGGGCC9720.12623852067155775No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT9220.11974476960820601No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9120.11844601939553567No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9090.11805639433173458No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT8790.11416014369372351No Hit
CATGTAGGCTGTGCTCGCGGATGTGTCCCTCGTAATGGTGACTCTGCCCT8430.10948464292811029No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8400.10909501786430917No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATA8170.10610789237516736No Hit
ACCTGCATATGAGTAGCAGTAATATTCAGCCTCGTCCTCAGCCTGGAGCC8060.10467926714122998No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCT8010.10402989203489481No Hit
GCCTACATGAAACTGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTA8010.10402989203489481No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)