FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704777
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17560.24915682549231885No Hit
ATCACATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA15890.22546138707704708No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15640.22191416575739562No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14580.20687394736207337No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14290.20275917063127769No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14120.2003470601339147No Hit
ATAATATACGGCCGTGTCCTCGGCTCTCAGGCTGGTCATTTGCAGATACA14110.20020517128112864No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14050.1993538381644123No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13600.19296883978903967No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13410.19027295158610455No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12550.1780705102465035No Hit
ATATAATAGTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA11350.16104384791217646No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10720.1521048501866548No Hit
GTATATTATTGTGCGAAAGTGTTTACGGTTTTTGGAGTGGCTATTCACGA10490.14884140657257544No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10360.1469968514863567No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGGTCATTTGCAGATACACCG9980.14160507508048645No Hit
GCCGTATATTATTGTGCGAAAGTGTTTACGGTTTTTGGAGTGGCTATTCA9800.1390510757303374No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9670.13720652064411865No Hit
GGGCTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT9380.13309174391332293No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9210.13067963341595992No Hit
CTCCAGAGACAGTTCCAAGAACACGGTGTATCTGCAAATGACCAGCCTGA9020.12798374521302483No Hit
GTATGTGATATCACCACGACCACTGATACCTGAGACCCACTCCAGCCCCT8830.1252878570100897No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8720.12372707962944307No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8610.12216630224879642No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8480.12032174716257768No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT8230.11677452584292622No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC7740.10982197205640933No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7670.10882875008690691No Hit
GTGTATCTGCAAATGACCAGCCTGAGAGCCGAGGACACGGCCGTATATTA7660.10868686123412087No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7640.10840308352854874No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7440.10556530647282758No Hit
ATATTACTGTCAGCAATATAATAGTCTCCCTCTCACTTTCGGCGGAGGGA7410.10513963991446941No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7400.10499775106168334No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7380.10471397335611124No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7360.1044301956505391No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7330.10400452909218093No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT7310.10372075138660881No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7280.10329508482825064No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG7220.10244375171153429No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAA7130.10116675203645975No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT7090.10059919662531554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12350.078.312471
TCAACGC14950.063.6187174
CAACGCA14950.063.61425
ATCAACG15050.063.1960033
AACGCAG15650.060.712796
TATCAAC15900.060.2707672
CGCAGAG19050.050.2511988
GCAGAGT20700.046.2456679
CAGCGCT4700.041.348119
GTACATG18500.037.4535141
TACATGG19300.034.3465162
ACATGGG19300.033.9731863
TACACGG8500.033.873626
GCAGCGC5600.033.4175878
AGCGCTG4750.033.3695565
GTACACG8650.033.3169485
GTATGTA9650.032.161351
TATGTAC9350.030.824822
GGCAGCG6100.030.678447
CATGGGA11300.030.6065924
Produced by FastQC (version 0.11.5)