FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704777
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16050.227731608721624No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15230.2160967227931672No Hit
ATCACATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA13870.19679983881426324No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13800.19580661684476083No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12930.1834622866523737No Hit
ATAATATACGGCCGTGTCCTCGGCTCTCAGGCTGGTCATTTGCAGATACA12920.18332039779958767No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12780.18133395386058285No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12240.1736719558101357No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12160.17253684498784722No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12000.17026662334327028No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11950.16955717907933998No Hit
ATATAATAGTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA10910.15480073838958988No Hit
GTATATTATTGTGCGAAAGTGTTTACGGTTTTTGGAGTGGCTATTCACGA10210.1448685186945658No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9220.130821522268746No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9170.13011207800481572No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGGTCATTTGCAGATACACCG9020.12798374521302483No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8920.12656485668516426No Hit
GCCGTATATTATTGTGCGAAAGTGTTTACGGTTTTTGGAGTGGCTATTCA8820.12514596815730367No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8680.12315952421829883No Hit
GTATGTGATATCACCACGACCACTGATACCTGAGACCCACTCCAGCCCCT8580.12174063569043825No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8540.12117308027929402No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8390.11904474748750313No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8380.11890285863471708No Hit
GGGCTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT8350.11847719207635891No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8060.11436241534556321No Hit
CTCCAGAGACAGTTCCAAGAACACGGTGTATCTGCAAATGACCAGCCTGA7680.10897063893969298No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT7670.10882875008690691No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7650.1085449723813348No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT7470.10599097303118576No Hit
ATATTACTGTCAGCAATATAATAGTCTCCCTCTCACTTTCGGCGGAGGGA7290.10343697368103669No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7240.1027275294171064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8100.051.5391041
AACCGCG450.00995104247.9437687
TCAACGC8850.046.2988134
ATCAACG8850.046.295523
AACGCAG9000.045.5465776
CAACGCA9200.044.5469365
GAATACG1851.8189894E-1242.7636959
TATCAAC9800.041.8076932
GTACATG17850.039.5167961
TACATGG18100.038.5212672
ACCGTCG750.001542711338.357748
ACATGGG18700.036.516523
CGCAGAG11900.035.658198
GCAGCGC4550.034.774878
CAGCGCT4300.033.451529
CATGGGA10550.032.7059674
GTGCTAG2705.456968E-1231.989791
GCAGAGT13650.031.086639
GGCAGCG5350.029.572797
GCTAGAG3201.8189894E-1229.2012083
Produced by FastQC (version 0.11.5)