FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285502
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33920.2638657893958936No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA31980.24877440875237844No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30540.2375725592025528No Hit
CCTATGTACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTCTC29900.23259395940263025No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC29790.23173826256201857No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC29680.2308825657214069No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA28160.2190583911965909No Hit
ATCCTATGTACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTC28120.21874722870909574No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC26660.2073897979155225No Hit
GTATTACTGTCAGCAGTTTGGTACCTCACCAGGAACGTTCGGCCAAGGGA26440.20567840423429914No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25810.2007775950562504No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22730.17681808351912326No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG22000.17113936812233665No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20170.1569036843194332No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20020.15573682499132635No Hit
GTACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTCTCAGTCA19590.1523918282507534No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT18790.14616857850085024No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA18090.140723234969685No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC17740.13800056320410237No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCT17300.13457777584165564No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC16790.13061045412609237No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA16700.12991033852922826No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16400.12757661987301458No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG16370.12734324800739322No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG15830.12314255442620858No Hit
ACGCAGACTCAGTGAAGGGCAGATTCACCATTTCCAGAGACAACTCCAAA15740.1224424388293445No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC15640.12166453261060658No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15340.1193308139543929No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT15280.11886407022315018No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14490.11271861109512081No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14420.11217407674200429No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14390.11194070487638291No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14270.11100721741389745No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGCCTGCTGATGGTGAGAGT14240.11077384554827609No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG14230.11069605492640229No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC14140.10999593932953819No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC13960.10859570813580997No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA13930.1083623362701886No Hit
CTCTGAGAGTCTCCTGTTCAGCCTCCGGACTCACCTTCAGCAACTATCCT13790.10727326756395557No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13740.1068843144545866No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGGCCCGGGATTCCCAGGTGT13660.1062619894795963No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13620.10595082699210114No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC13580.10563966450460598No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG13450.10462838642024673No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC13360.1039282708233826No Hit
CTACGCAGACTCAGTGAAGGGCAGATTCACCATTTCCAGAGACAACTCCA13210.10276141149527579No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGGCTGGAGCCTGAAGATT13010.10120559905779998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)