FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285502
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32450.25243056798044655No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA29750.23142710007452344No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC29010.225670594055863No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC27070.2105792134123479No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27010.21011246968110514No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA26550.20653410107491083No Hit
GTATTACTGTCAGCAGTTTGGTACCTCACCAGGAACGTTCGGCCAAGGGA26370.2051338698811826No Hit
CCTATGTACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTCTC26170.20357805744370683No Hit
ATCCTATGTACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTC26130.20326689495621164No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23720.1845193550846284No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22900.1781405240909777No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20330.1581483342694138No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT20000.15558124374757878No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG19110.14865787840081152No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18790.14616857850085024No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA18600.14469055668524824No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT17710.137767191338481No Hit
GTACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTCTCAGTCA17590.13683370387599553No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT17370.13512231019477217No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17360.13504451957289837No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17010.13232184780731573No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCT16070.12500952935117954No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15850.12329813566995618No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15710.12220906696372312No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG15300.11901965146689775No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC15070.11723046716380059No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC14780.11497453912946072No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14470.11256302985137324No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGCCTGCTGATGGTGAGAGT14220.11061826430452851No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC14210.11054047368265472No Hit
ACGCAGACTCAGTGAAGGGCAGATTCACCATTTCCAGAGACAACTCCAAA13810.10742884880770313No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13640.10610640823584873No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA13420.10439501455462535No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGGCCCGGGATTCCCAGGTGT13320.10361710833588746No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGGCTGGAGCCTGAAGATT13050.10151676154529514No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG12900.1003499022171883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15300.065.378551
ATCAACG16300.058.6522943
TCAACGC16850.056.744464
CAACGCA17750.053.4685065
TATCAAC18950.051.5882342
GGTATCA7400.051.5413781
AACGCAG18550.050.786876
CGCAGAG21500.043.821858
GAATACG2650.037.996199
GTACATG31750.036.265041
GCAGAGT27050.034.8306779
TACATGG31700.034.69392
GTATGTA17050.033.7658351
CATGGGA19600.033.377754
ACTATCG900.00377381531.965058
ACATGGG34250.031.9009783
GCAGCGC10100.031.3320778
CAGCGCT9750.030.2438559
TACACGG15550.030.0612496
GGCAGCG9900.029.7832937
Produced by FastQC (version 0.11.5)