FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837437
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28740.3431899951876977No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20490.24467512183006007No Hit
GTGTGTACCTAGCATGCAGTAATAAAGTCCAACATCCTCAGCCTCCACCC20190.24109276279887323No Hit
TTATTACTGCATGCTAGGTACACACTGGCCTCCGATGTACACTTTTGGCC18710.22341979157835157No Hit
GGTACACACTGGCCTCCGATGTACACTTTTGGCCAGGGGACCAAGCTCGA17490.20885153151819183No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15010.17923736352704742No Hit
GTATGTGAGATCTCCACTAGCACTAATACTTGAGACCCACTCCAGCCCCT14010.16729616675642467No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13770.1644302795314752No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13540.16168380427423196No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13520.16144498033881952No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13200.15762379737222024No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12580.15022025537443415No Hit
AGGTACACACTGGCCTCCGATGTACACTTTTGGCCAGGGGACCAAGCTCG12190.14556318863389126No Hit
TTTATAAGGTTTCTAGCCGGGACTCTGGGGTCCCAGACAGATTCAGCGGC11930.14245847747352935No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11900.14210024157041068No Hit
GTAATAAAGTCCAACATCCTCAGCCTCCACCCTGCTGATCGTCAGTGTGA11580.1382790586038114No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTATCAGCAGAGGCCAG11300.13493552350803703No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT11040.1318308123476751No Hit
GAAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAAAAACA10950.13075610463831905No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAA10870.12980080889666923No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10730.12812904134878206No Hit
CAGTAATAAAGTCCAACATCCTCAGCCTCCACCCTGCTGATCGTCAGTGT10230.12215844296347068No Hit
CTCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCCTCTCCAGAGA10170.12144197115723332No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA10140.12108373525411464No Hit
GTCTGGGACCCCAGAGTCCCGGCTAGAAACCTTATAAATTAGGCGCCTTG10100.12060608738328972No Hit
GTGATGGAAACACCTACTTGAATTGGTATCAGCAGAGGCCAGGCCAATCT10030.11977020360934612No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9920.11845667196457763No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTATCAGCAGAGGCC9860.11774020015834026No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC9640.11511313686880327No Hit
GGTATCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTT9620.11487431293339082No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9620.11487431293339082No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9530.11379960522403475No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9380.11200842570844136No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9110.1087843025803732No Hit
CTCCAGAGACAATTCCATGAACACGCTGTTTTTGCAAATGAACAGCCTGA9090.10854547864496075No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC9000.1074707709356047No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8990.10735135896789848No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA8900.10627665125854244No Hit
GATTTCACACTGACGATCAGCAGGGTGGAGGCTGAGGATGTTGGACTTTA8900.10627665125854244No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA8760.10460488371065524No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8750.10448547174294902No Hit
TTCCATCACTGTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCC8660.10341076403359298No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8480.10126134861488088No Hit
CTTTATTACTGCATGCTAGGTACACACTGGCCTCCGATGTACACTTTTGG8380.10006722893781861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG12200.044.866343
TCAACGC12750.042.928374
TACATGG22100.040.413112
GTACATG22650.040.0725631
TATCAAC13750.039.811062
CAACGCA13950.039.233265
GTATCAA14750.039.069941
CGCAGAG14300.038.2684338
AACGCAG14550.037.6108976
GAATACG3050.035.4081049
CATGGGG12550.033.2832154
ACATGGG28550.031.0288683
GTATTAC2351.3515091E-930.6532761
GCAGAGT18350.029.8187059
GTTATAC1306.7304325E-427.7058471
TACGAAG1306.742201E-427.697575
CTAGAGA4500.025.6063964
TCTACAC9200.025.0512333
GCTAGAG4900.024.9873263
GTCTATA1450.00127311924.8397251
Produced by FastQC (version 0.11.5)