FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018356
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT73900.3661395710172041No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC57920.28696622399616317No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGTCTGGAGTGGGTTG48340.23950185200232268No Hit
CTCATAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCA46280.22929552566544256No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT42680.21145922721264235No Hit
CCTTGATAGTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCG42570.21091422920436237No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT42200.20908105408560235No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT39240.19441565313552217No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37760.1870829526604821No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG36060.178660256168882No Hit
ATAATACACGGCCGTGTCCTCGGTTTTCAAACTGTTGATTTGCAGGTACA35770.177223443237962No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35010.17345800245348195No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG34840.17261573280432194No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA31630.15671170001724175No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGCGG31380.15547306818024176No Hit
GTATTATTGTGCTAGAGGACATGGCAGTGACTGGCGAGACCTTGACGTCT31370.15542352290676176No Hit
CTATCAAGGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT30810.1526489875918817No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT30580.1515094463018417No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30020.14873491098696168No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT29170.14452356274116163No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC29170.14452356274116163No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT28270.14006448812796157No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG27880.13813222246224155No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT27340.13545677769432152No Hit
GTACATGGGAGCTCTGAGAGCGGAGCCCCAGCCCCAGAATTCCCAGGTGT26830.1329299687468415No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26260.13010588815848145No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG26250.13005634288500145No Hit
GAATATGCCCCGTCTATGAAAGGCAGATTCACCATCTCAAGGGATGGTTC25810.12787635085188143No Hit
TATTTAGCCTGGTATCACCAAAAACCAGGAAAAGCCCCTAAGCTCCTGAT25810.12787635085188143No Hit
ACTTATTACTGTCAACACCTTGATAGTTACCCGCTCACTTTCGGCGGAGG25240.1250522702635214No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA24950.12361545733260138No Hit
CAATAATACACGGCCGTGTCCTCGGTTTTCAAACTGTTGATTTGCAGGTA24590.12183182748732137No Hit
ATCTGGGACAGAGTTCACTCTCATAATCAGCAGCCTGCAGCCTGAAGATT24530.12153455584644136No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23530.1165800284984413No Hit
GTGTATTATTGTGCTAGAGGACATGGCAGTGACTGGCGAGACCTTGACGT23490.1163818474045213No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23220.11504412502056129No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22680.11236868025264125No Hit
GTACATGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCT22580.11187322751784125No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC22480.11137777478304124No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA22440.11117959368912124No Hit
GATTATGAGAGTGAACTCTGTCCCAGATCCACTGCCGCTGAATCTTGATG22160.10979232603168124No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG22100.10949505439080123No Hit
GAGTTCACTCTCATAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA21640.10721597181072119No Hit
GTTCAGAGAACACACTGTACCTGCAAATCAACAGTTTGAAAACCGAGGAC21600.1070177907168012No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA21400.10602688524720119No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT21210.10508552505108118No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT21140.10473870813672116No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC20940.10374780266712115No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC20650.10231098973620115No Hit
TTATTACTGTCAACACCTTGATAGTTACCCGCTCACTTTCGGCGGAGGGA20630.10221189918924116No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20460.10136962954008114No Hit
TCTCTGAACCATCCCTTGAGATGGTGAATCTGCCTTTCATAGACGGGGCA20350.10082463153180113No Hit
CTCCTGTGCAGCCTCTGGATTCACCCTCAGTGAGCACTACATGGACTGGG20330.10072554098484114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29050.081.695011
ATCAACG36650.064.46613
TCAACGC36800.064.203334
CAACGCA38050.062.0926175
AACGCAG39950.059.0955776
TATCAAC41250.057.8010372
ACGCAGA45750.051.6023987
CGCAGAG47950.049.234828
GCAGAGT54850.042.9099739
GTACATG55900.039.8742371
ACATGGG57750.037.544483
GTACACG18800.035.2493255
TATGTAC19400.034.1599852
GTATGTA20650.033.8842121
AAGCGCT7500.033.6154064
AGCGCTG8300.032.1103065
ACACGGG20400.032.10697
TACATGG93350.031.25162
ACGGGGT21550.030.0595449
CACGGGG23100.028.0425628
Produced by FastQC (version 0.11.5)