FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018356
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT70230.3479564556500439No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52350.2593695066678029No Hit
CTCATAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCA42040.20828832970992234No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGTCTGGAGTGGGTTG41700.20660379041160232No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT38950.19297884020460218No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38660.19154202727368216No Hit
CCTTGATAGTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCG37820.1873802243013621No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT35500.17588572085400198No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG33690.16691802635412187No Hit
ATAATACACGGCCGTGTCCTCGGTTTTCAAACTGTTGATTTGCAGGTACA33050.16374712885140183No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32190.15948623533212178No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA31420.15567124927416176No Hit
GTATTATTGTGCTAGAGGACATGGCAGTGACTGGCGAGACCTTGACGTCT31370.15542352290676176No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30960.1533921666940817No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29650.14690173586820163No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT29440.14586128512512164No Hit
CTATCAAGGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT29430.14581173985164164No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGCGG29370.14551446821076164No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT29250.14491992492900163No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT28040.13892494683792156No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC27920.13833040355616155No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG27340.13545677769432152No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT25830.12797544139884146No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25560.1266377190148814No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG24850.12312000459780137No Hit
GTACATGGGAGCTCTGAGAGCGGAGCCCCAGCCCCAGAATTCCCAGGTGT23990.11885911107852133No Hit
GAATATGCCCCGTCTATGAAAGGCAGATTCACCATCTCAAGGGATGGTTC23830.11806638670284132No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23820.11801684142936132No Hit
GATTATGAGAGTGAACTCTGTCCCAGATCCACTGCCGCTGAATCTTGATG23720.11752138869456132No Hit
ACTTATTACTGTCAACACCTTGATAGTTACCCGCTCACTTTCGGCGGAGG23060.11425140064488126No Hit
CAATAATACACGGCCGTGTCCTCGGTTTTCAAACTGTTGATTTGCAGGTA23040.11415231009792128No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA23000.11395412900400129No Hit
GTGTATTATTGTGCTAGAGGACATGGCAGTGACTGGCGAGACCTTGACGT22930.11360731208964127No Hit
GAGTTCACTCTCATAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA22850.11321094990180128No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA22790.11291367826092126No Hit
TATTTAGCCTGGTATCACCAAAAACCAGGAAAAGCCCCTAAGCTCCTGAT22780.11286413298744126No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22670.11231913497916125No Hit
ATCTGGGACAGAGTTCACTCTCATAATCAGCAGCCTGCAGCCTGAAGATT22200.10999050712560124No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC22150.10974278075820124No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA22060.10929687329688123No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA21880.1084050583742412No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG21710.10756278872508121No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21560.1068196096228812No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT21500.1065223379820012No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20970.10389643848756117No Hit
TTATTACTGTCAACACCTTGATAGTTACCCGCTCACTTTCGGCGGAGGGA20700.10255871610360115No Hit
GTACATGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCT20670.10241008028316115No Hit
GTTCAGAGAACACACTGTACCTGCAAATCAACAGTTTGAAAACCGAGGAC20570.10191462754836114No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC20300.10057690516440113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20500.070.587251
TCAACGC24350.059.0620424
ATCAACG25200.057.6334233
CAACGCA25600.056.189315
AACGCAG27700.052.716266
TATCAAC28450.051.0483742
ACGCAGA31300.046.4232257
CGCAGAG33300.043.636138
GTACATG52600.040.9231951
ACATGGG52450.039.6164863
GCAGAGT38400.038.0280349
GTATGTA21600.031.3298421
TACATGG88450.030.8073392
AAGCGCT7350.029.350224
GTACACG21050.028.700595
TATGTAC21050.028.6906242
ACACGGG22250.027.1567367
AGCGCTG9050.027.0205385
ACGGGGT22450.026.9154749
CATGGGG30000.026.8456694
Produced by FastQC (version 0.11.5)