FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633199
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGAAACA59250.36278493925112615No Hit
GTTTATTACTGTGCGAGAGAACATTGTAGTGGCGCCAACTGCTATCTCTA58520.35831518388144984No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT54570.33412952126470813No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39130.2395911337197733No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT39020.2389176089380412No Hit
CTCCTGATCCATGCTGCATCCACTTTGCGACATGGGGTCCCATCGAGGTT35530.21754850449945168No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA30320.1856479216555974No Hit
GACTTACAGTACCCGATACACTTTTGGCCAGGGGACCAAGCTGGAGATCA30300.1855254626043734No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC30140.1845457901945813No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30100.1843008720921333No Hit
GTAGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCAGACCATTAGCAT30010.18374980636162525No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA29640.18148431391398107No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28170.17248357364901643No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC27910.17089160598310432No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT24260.14854282913472272No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT23120.1415626632149542No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22930.14039930222832614No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT22600.13837872788313No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22510.13782766215262196No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22440.13739905547333792No Hit
CACCATCAGTAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC22400.13715413737088988No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22340.13678676021721786No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA21900.13409266109028967No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG21340.13066380765601743No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT21330.1306025781304054No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21180.12968413524622535No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21150.12950044666938934No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT21090.12913306951571732No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG20280.12417347794114496No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG19890.12178552644227679No Hit
CTCCAGAGACAACGCCAGGAACTCACTGTTTCTGCAAATGAACAGCCTGA19040.11658101676525641No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18990.1162748691371964No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18880.11560134435546433No Hit
GATCAGGAGCATAGGGGCCTTCCCTGGTTTCTGTTGATACCAATTTAAAT18150.11113158898578802No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTT17910.1096620803710999No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17690.10831503080763583No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17550.10745781744906774No Hit
CTCCTGTGCAGCCTCTGGATTCAGCTTTAGTAGTTACTGGATGAGCTGGG17300.10592707930876764No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC17160.10506986595019958No Hit
CCATCAGTAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG16940.10372281638673549No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA16910.10353912780989946No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16540.10127363536225531No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16430.10060011058052325No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)