FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834444
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA38140.45707081601641336No Hit
TTATAGTACTCCTCCCGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC26820.32141162258941286No Hit
ATATTATAGTACTCCTCCCGCGTTCGGCCAAGGGACCAAGGTGGAAATCA24300.29121187281591093No Hit
GTATATTACTGTGCGAAGGCTAAGGGTTACGATTTTTGGAGTGGTTACCC23420.2806659284505611No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA22770.27287631045342764No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21060.2523836231071228No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCG17010.20384831097113765No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16710.20025310266476842No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14280.1711319153831773No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13700.1641811793241967No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAG13240.15866852658776381No Hit
GTATGTGCTACCACCACTACCACTAATAGCTGAGACCCACTCCAGCCCCT12670.15183763080566223No Hit
GCCGTATATTACTGTGCGAAGGCTAAGGGTTACGATTTTTGGAGTGGTTA12640.1514781099750253No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12550.15039954748311452No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT12300.1474035405611401No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA12040.14428769336228675No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11910.14272976976286006No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA11700.14021312394840157No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11480.13757663785706412No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11380.13637823508827435No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10990.1317044642899943No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT10980.1315846240131153No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10900.1306259017980835No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10800.12942749902929374No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10800.12942749902929374No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10710.12834893653738297No Hit
TTATTACTGTCAGCAATATTATAGTACTCCTCCCGCGTTCGGCCAAGGGA10680.12798941570674605No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10170.1218775615859183No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9880.11840219355642799No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTA9710.11636490884948542No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT9520.11408794358878487No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9480.11360858248126897No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9320.11169113805120534No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA9280.11121177694368946No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT9170.10989353389802073No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG9090.10893481168298891No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT8730.10462056171534578No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8580.10282295756216114No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCTCCCGCGTTCGGCCAAGG8520.1021039159008873No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8420.10090551313209754No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8380.10042615202458165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19800.055.292631
ACACCGG553.3295638E-452.36686
TCAACGC22500.046.722824
ATCAACG22550.046.619223
CAACGCA23100.045.509245
AACGCAG23600.044.8501636
TATCAAC24100.043.9196622
GGTATCA11150.040.6962661
TATACGG15400.040.2102175
CGCAGAG26650.039.4423078
TCTAGTC1105.517133E-639.27512
ATACGGC15650.039.1077886
TACGGCC15500.039.0170337
AATATAC16700.037.5112463
ATATACG16950.036.9579854
GCAGAGT30200.035.282679
GTAATAT17800.035.2036821
GACCGCG1051.9310496E-434.2836727
TAATATA18550.033.7702332
GTACATG27050.031.6860831
Produced by FastQC (version 0.11.5)