FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834444
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA39310.4710921284112535No Hit
TTATAGTACTCCTCCCGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC26520.3178164142830436No Hit
ATATTATAGTACTCCTCCCGCGTTCGGCCAAGGGACCAAGGTGGAAATCA24540.29408803946100637No Hit
GTATATTACTGTGCGAAGGCTAAGGGTTACGATTTTTGGAGTGGTTACCC23360.27994688678928725No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAG21660.25957403971986137No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20500.2456725676019002No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT17250.20672447761623308No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA16950.2031292693098638No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16580.1986951790653417No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCG15880.19030635968381343No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15470.1853929083317754No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13750.16478038070859158No Hit
GTATGTGCTACCACCACTACCACTAATAGCTGAGACCCACTCCAGCCCCT12910.15471379745075764No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12700.15219715163629915No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA12550.15039954748311452No Hit
GCCGTATATTACTGTGCGAAGGCTAAGGGTTACGATTTTTGGAGTGGTTA11430.13697743647266922No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11430.13697743647266922No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11280.13517983231948458No Hit
TTATTACTGTCAGCAATATTATAGTACTCCTCCCGCGTTCGGCCAAGGGA11020.13206398512063122No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA10910.1307457420749625No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10880.13038622124432558No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10690.12810925598362502No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10360.12415452684661882No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10190.12211724213967623No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10040.12031963798649162No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9990.11972043660209673No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTA9880.11840219355642799No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA9670.1158855477419695No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9620.11528634635757462No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT9350.11205065888184229No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9020.10809592974483609No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG8920.10689752697604633No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGG8850.1060586450378935No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8710.10438088116158782No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT8700.10426104088470886No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA8650.10366183950031398No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8630.10342215894655603No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8620.10330231866967705No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8560.10258327700840321No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT8520.1021039159008873No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCTCCCGCGTTCGGCCAAGG8390.10054599230146062No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTCG450.00990453948.001445
TAGCGAC450.00991157847.992817
GTATCAA13000.044.884411
ATCAACG12700.043.6627043
TCAACGC13100.042.321884
CAACGCA13600.040.765935
TATACGG17900.039.017935
ATACGGC17900.038.608746
AACGCAG14500.038.2287566
TACGGCC18350.037.661937
TATCAAC14950.037.0980722
AATATAC19600.035.640133
CGCAGAG15650.035.419618
ATATACG20050.034.833964
GTAATAT20650.034.535761
GTATTAG3400.033.8996281
CATGGGG12950.033.3600734
TAATATA21350.033.0620652
ACATGGG22350.032.8659553
GTACATG22850.032.786951
Produced by FastQC (version 0.11.5)