FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673164
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC78260.46773657573316185No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT55440.33134827189683735No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCA54800.327523183620972No Hit
GACCTAGGATGGTGACCTTGGTCCCAGTTCCAAAGACGAGAGCGCTGGTG51690.30893564528043876No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCGGTGACGTT45450.27164103459075145No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA44620.26668037323298854No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC44310.26482759609936624No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT40520.242175901465726No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT39410.23554176398727203No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC39110.23374875385796012No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA38340.22914669452605962No Hit
GATTATTACTGCAGCTCATATAGAAGCACCAGCGCTCTCGTCTTTGGAAC36920.2206597799139833No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG36720.21946443982777541No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC35410.21163496226311346No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34630.20697313592690256No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33930.20278944562517484No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC33890.20255037760793324No Hit
ATCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC33100.19782878426741193No Hit
GTCTAACTCGGGGACCCCTGGGTGATGCTTTTGATATCTGGGGCCAAGGG32250.19274858890102822No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC32180.19233021987085547No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT31710.18952117066826682No Hit
CTCCTGCACTGGAACCAGCGGTGACGTTGGTGGTTATAACTATGTCGCCT31420.18778792754326534No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT31050.18557654838378068No Hit
ACCTAGGATGGTGACCTTGGTCCCAGTTCCAAAGACGAGAGCGCTGGTGC30900.18468004331912471No Hit
GGTTATAACTATGTCGCCTGGTACCAACACCACCCAGGCAAAGCCCCCAA30620.18300656719843364No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT30540.18252843116395046No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30330.18127332407343213No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT30280.18097448905188016No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG28710.17159106937514793No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG28640.17117270034497514No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA28520.1704554962932504No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA28510.17039572928893998No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA28450.17003712726307763No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC28450.17003712726307763No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27780.16603273797428106No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG27710.1656143689441083No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG26690.15951813450444788No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT25990.15533444420272013No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC25810.154258638125133No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT25300.15121052090530276No Hit
GTGTTGGTACCAGGCGACATAGTTATAACCACCAACGTCACCGCTGGTTC25170.1504335498492676No Hit
GCCTTGGGCTGACCTAGGATGGTGACCTTGGTCCCAGTTCCAAAGACGAG24300.14523382047426314No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT23370.13967548907339628No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC22920.13698597387942843No Hit
TAACTATGTCGCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAACTCA22750.1359699348061517No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT22330.13345972062511505No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22230.1328620505820111No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG22210.13274251657339028No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA22140.1323241475432175No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT21540.12873812728459375No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21440.12814045724148976No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT21090.1260486120906259No Hit
GAAATAGACGGCCGTGTCTTCGGCTCTCAGGCTGTCCATTTGCAGAGACA20790.12425560196131402No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG20680.12359816491389966No Hit
GAGTTAGACAGGCCAAAACCGCACAGAAATAGACGGCCGTGTCTTCGGCT20280.1212074847414838No Hit
GGACTGAACAGAGAGAACTCACCATGGGGTTTGGGCTGACCTGGCTTTTT20150.12043051368544865No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG20040.11977307663803428No Hit
GTCTATTTCTGTGCGGTTTTGGCCTGTCTAACTCGGGGACCCCTGGGTGA20010.1195937756251031No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA19600.11714332844837685No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCGGTGACGTTGGTGGTTATA19510.11660542540958328No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG19370.11576868734923774No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC19350.11564915334061694No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT19240.11499171629320258No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG18800.11236196810354512No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT18660.11152523004319959No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA18450.11027012295268127No Hit
TCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGC18310.10943338489233573No Hit
CCCCTGGGTGATGCTTTTGATATCTGGGGCCAAGGGACACTGCTCACCGT18270.10919431687509414No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18250.10907478286647333No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18190.10871618084061095No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCCATGAGCTGGG18170.10859664683199018No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCC17870.10680363670267827No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17600.10518992758629757No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC17310.10345668446129608No Hit
GTTATAACCACCAACGTCACCGCTGGTTCCAGTGCAGGAGATGGTGATCG17200.10279924741388173No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC17020.1017234413362946No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT16880.10088670327594904No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG16850.10070740226301785No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG16760.10016949922422429No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8900.085.7948461
GTATCAA26250.085.070251
ATCAACG33800.065.823263
TATCAAC33950.065.532432
TCAACGC33950.065.320364
CAACGCA35700.062.320075
AACGCAG37750.058.743316
CGCAGAG42500.052.0084728
GCAGAGT47800.046.3924679
GAATACG2150.040.1853189
GTACATG45450.036.7704931
CATGGGA16500.036.654984
TACATGG45450.036.2776572
ACATGGG46650.035.3444673
ATGGGAG15700.032.5608985
GGATATT10300.031.4717311
CAGAGTA47400.030.5310949
GTACACG17350.029.4643255
GTGCTAG2951.6370905E-1129.3024921
TATACGG1000.006284154528.8003435
Produced by FastQC (version 0.11.5)