FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779112
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATACACTGGGTGCGACAGGCCCCTGGACACGGGCTTGAGTGGATGG47940.2694602700673145No Hit
ACTATATACACTGGGTGCGACAGGCCCCTGGACACGGGCTTGAGTGGATG45720.25698213490775174No Hit
GTAATAAACGGCCGTGTCGTCGGAGGACAGTCTGCTCACCTCCATGTAGG42390.23826493216840763No Hit
GTATTACTACTACTACCCCACTTTGGACGTCTGGGGCCAAGGGACCACGG36550.2054395676045128No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32180.1808767519976258No Hit
GTTTATTACTGTGCGAGAGATGTGGGCTCAAACTTGGACTACAGTGCCAA28350.15934915845657835No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27800.15625772857470469No Hit
GTGTATATAGTAGCCGATGAAGGTGTAACCAGAAGCCTTGCAGGAGACCC27310.15350354558903542No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCGGTGAGGGTCTCCTGCA25400.14276785272652873No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24280.1364725773307133No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23520.13220078331212426No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21150.11887953091205052No Hit
GTCCAGGGACACGTCCATCAGTACAGCCTACATGGAGGTGAGCAGACTGT21120.11871090746394831No Hit
GTATATAGTAGCCGATGAAGGTGTAACCAGAAGCCTTGCAGGAGACCCTC21090.1185422840158461No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20730.11651880263861972No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19510.1096614490824636No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19000.10679485046472623No Hit
GTTATAGACTCCCTTACACTTTTGGCCTGGGGACCAAGCTGGAGCTCAGT18940.10645760356852182No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18540.10420929092715916No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17900.10061199070097891No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32900.073.7495651
TCAACGC39150.061.2455524
ATCAACG39550.060.6261333
TATCAAC42950.055.323912
CAACGCA43600.054.662745
AACGCAG44500.053.2365846
CGCAGAG50200.047.048358
ACGCAGA56750.041.6181037
GCAGAGT56850.041.1591269
TAAACGG21450.033.23215
GGTATCA31300.031.7438961
GTACATG43150.031.7028471
TACACCG3750.030.7212285
AAACGGC23550.030.270436
ATAAACG23800.030.2541624
AACGGCC24300.030.225137
ACATGGG43400.028.7024153
CAGAGTA60350.025.7685369
CAGCGCT9150.025.1792139
CTATATA36300.024.3962971
Produced by FastQC (version 0.11.5)