FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572953
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCAGATTTCAGGCTGCTCAGTTCCATGTAGA36440.23166617184365965No Hit
ATATATTACTGTGCGAGAACGGAGGCTAACGCAGTGCCTACTGTGCCTCT30980.19695439088135502No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA23710.15073559095535594No Hit
ATATATGGCCGTGTCCTCAGATTTCAGGCTGCTCAGTTCCATGTAGACTG18460.11735887849160147No Hit
GCCATATATTACTGTGCGAGAACGGAGGCTAACGCAGTGCCTACTGTGCC18190.11564236185060837No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17750.11284507547269372No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16740.10642403174157143No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16720.10629688236075713No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26250.066.956431
TCAACGC29650.058.5254254
ATCAACG31250.055.528923
CAACGCA31850.054.707195
TATCAAC34400.050.6518552
GGTATCA19350.039.8322261
GCAGAGT46150.037.911689
AACGCAG51100.034.380126
CGCAGAG51800.033.7765248
GTACATG41900.032.664151
ACGCAGA54400.032.1622057
ACATGGG42850.029.7423083
CAGAGTA44300.024.5420959
CTGTGCG25200.024.2860479
TACATGG57050.023.6006742
TACACGG8850.023.5935446
GTAATAT26050.022.9510271
TTACGCT1902.2984858E-422.7378774
GTACACG9300.022.451925
CATGGGG28650.022.367514
Produced by FastQC (version 0.11.5)