FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572953
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCAGATTTCAGGCTGCTCAGTTCCATGTAGA33410.2124030406502928No Hit
ATATATTACTGTGCGAGAACGGAGGCTAACGCAGTGCCTACTGTGCCTCT30530.1940935298130332No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA21020.13363399923583222No Hit
GCCATATATTACTGTGCGAGAACGGAGGCTAACGCAGTGCCTACTGTGCC17170.10915774342907894No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16490.10483466448139263No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16060.10210095279388513No Hit
ATATATGGCCGTGTCCTCAGATTTCAGGCTGCTCAGTTCCATGTAGACTG15850.10076588429533495No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26700.074.705921
ATCAACG31050.062.3726653
TCAACGC31400.061.677434
CAACGCA33550.057.5103385
TATCAAC34650.056.1001662
GGTATCA19300.042.1606831
GCAGAGT46400.040.9614949
AACGCAG53900.035.797256
CGCAGAG53700.035.6624348
ACGCAGA56000.034.3262947
GTACATG43350.031.2287961
ACATGGG44750.028.476323
CAGAGTA43250.024.635739
TACACGG8900.024.2680516
GTAATAT24900.023.1354061
TAACCGT2202.3536038E-522.907577
TACATGG60050.022.659612
ATATGGC25800.022.603156
CATGGGA23050.022.4887434
TATGGCC24100.022.4051517
Produced by FastQC (version 0.11.5)