FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2080337
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTC44310.21299433697521122No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44230.21260978389559No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28450.1367566889403015No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTTTCGTCCCTCCGCCGAATATCTT25400.12209560277974194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23620.11353929675816947No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48650.074.4861451
GGTATCA20700.070.6506351
TCAACGC55750.063.804774
ATCAACG56400.063.1970983
CAACGCA59650.059.6273885
TATCAAC60000.059.405272
AACGCAG63500.056.0121846
ACGCAGA72200.049.0633547
CGCAGAG72650.048.759458
GCAGAGT86000.041.2741179
GTACATG61700.029.7745591
ACATGGG59850.029.1153683
TACATGG63700.027.8078082
CAGAGTA82750.025.493479
GTATATA17950.024.0811441
TAGACCG1500.001556346123.9998255
CATGGGG42650.023.9739934
GTCGTAC2151.96065E-523.4557821
GTACACT7950.021.737784
TATTGAG25050.019.2573435
Produced by FastQC (version 0.11.5)