FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022772
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACACTGGTACCAGCAGCTTCCCGGAACAGCCCCCAAACTCCTCAT49450.2444665043811166No Hit
CCTTATGATATACACTGGTACCAGCAGCTTCCCGGAACAGCCCCCAAACT47880.2367048782561752No Hit
GTAATATACAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGCAGAAACA34470.17040971498517876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30300.14979444050046176No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC29720.14692708817405026No Hit
ATGATATACACTGGTACCAGCAGCTTCCCGGAACAGCCCCCAAACTCCTC28850.14262605968443304No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG28070.13876996517650037No Hit
TCCTATGACAGTAGCCTGGGTGGTGTGGTATTCGGCGGAGGGACCAGGCT24250.11988498950944546No Hit
GTATATTACTGTGCGACGAGAGGAGGGGGGGGAAAGGAGACAGTAGTGGT24210.1196872410731412No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATACCACACCACCCAGG23580.11657270320134944No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATACCAC21170.10465835991401898No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC20960.10362018062342172No Hit
CTCCTGCACTGGGAGCAGCTCCCACATCGGGTCACCTTATGATATACACT20380.10075282829701024No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40000.081.913191
GGTATCA17300.066.600331
TCAACGC50300.064.7022254
ATCAACG51050.063.4695633
TATCAAC53350.060.8682562
CAACGCA55850.057.8857845
AACGCAG58500.055.2636076
CGCAGAG66400.048.146388
GCAGAGT72750.043.7416429
ACGCAGA81100.039.5082637
GTACATG57900.033.829281
TACATGG60300.032.359422
ACATGGG60450.031.802683
TATACAG27600.031.305525
CAGCCGT22850.029.3023579
AATATAC26000.029.078013
GTAATAT25250.028.8046381
CATGGGG43900.026.2424414
CAGAGTA71250.025.5647459
TAATATA28900.025.1635722
Produced by FastQC (version 0.11.5)