FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1845991
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCGTCAGACCTCAGGTTCCTCAATTCCATGTAGA34370.18618725660092603No Hit
GTATATTACTGTGCGAGAGATCTTGGGACCCCCAATTACCTTGAAACCTA24460.13250335456673407No Hit
GTCTACATGGAATTGAGGAACCTGAGGTCTGACGACACGGCCGTATATTA18760.1016256308941918No Hit
CCTTGGGAGAGTCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG18580.10065054488347994No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20550.050.7933354
CAACGCA21900.047.9909445
ATCAACG21800.047.880873
GTATCAA23750.046.720531
AACGCAG23550.044.0171746
TATCAAC26350.040.705792
ACGCAGA26100.039.7166447
CGCAGAG27800.036.770048
TATACGG20100.033.3071485
ATACGGC21450.030.8752946
GCAGAGT35600.029.320319
GTACATG43600.026.6066441
ATATACG25550.026.484244
GTAATAT30100.024.8953111
AATATAC31000.023.9180683
GGTATCA22350.023.5340161
TAATATA31900.023.4689252
GTATATA11100.023.3684671
TTCCGCG1952.7447834E-422.1496667
CTAGGTC11850.021.8692913
Produced by FastQC (version 0.11.5)