FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038520
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAGATTCCAGGCTGCTCAGCGCCATATAAA49970.4811655047567693No Hit
GTCTATTACTGTGCGAAAGGGCCCCCCTTCAAATATGATAGTAGTGGTTA39200.37746023186842814No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27170.26162230867003045No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT24590.23677926279705735No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23970.23080922851750565No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC23920.2303277741401225No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA23070.2221430497246081No Hit
CCTATGTGGTGACTCAGACACCCTCAGTGTCAGTGGCCCCAGGCAAGACG22410.21578785194314984No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22280.21453607056195356No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20530.19768516735354158No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19300.18584138966991487No Hit
GTCTGGGGCTGAATTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA18440.17756037437892386No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG18430.17746408350344722No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17950.1728421214805685No Hit
CTTTTTATATGGCGCTGAGCAGCCTGGAATCTGAGGACACGGCCGTCTAT17830.17168663097484882No Hit
GCCTCTGTGACCTCCTATGTGGTGACTCAGACACCCTCAGTGTCAGTGGC17740.17082001309555905No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17590.16937564996340948No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17500.16850903208411971No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16980.16350190655933441No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA16560.15945768978931554No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC16500.1588799445364557No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16400.15791703578168934No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT16180.15579863652120324No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15980.15387281901167046No Hit
CCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGGTGACTCAGACAC15920.15329507375881063No Hit
CCCCTGTGCTGGTCATCTATTACGATAGCGACCGGCCCTCAGGGATCCCT15630.15050263836998806No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15420.14848052998497863No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA15230.1466510033509225No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA14480.13942918769017448No Hit
TGATAGTAGTGGTTACCACAACTGGTTCGACCCCTGGGGCCAGGGAACTC14300.13769595193159498No Hit
CCCTCAGTGTCAGTGGCCCCAGGCAAGACGGCCAGAATTACGTGTGGGGG14120.13596271617301545No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG14120.13596271617301545No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14080.13557755267110888No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13950.13432577128991258No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA13790.13278511728228634No Hit
GCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCGCCGCCGAACACCAG13770.13259253553133304No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG13750.13239995378037978No Hit
CACCAGCACTATCCCACATCTGACAGTAATAGTCGGCCTCATCCCCGGCC13670.13162962677656667No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC13380.1288371913877441No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG13310.12816315525940764No Hit
GTGCTGGTGATCATCTGGTGTTCGGCGGCGGGACCAAGCTGGCCGTCCTA13240.12748911913107114No Hit
GACCTAGGACGGCCAGCTTGGTCCCGCCGCCGAACACCAGATGATCACCA13210.1272002465046412No Hit
ATCCCACATCTGACAGTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGC13010.12527442899510843No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13010.12527442899510843No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT12800.123252320610099No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12710.12238570273080923No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12660.12190424835342602No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12660.12190424835342602No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12650.1218079574779494No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12630.12161537572699611No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT12600.12132650310056618No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12510.12045988522127644No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12440.11978584909293996No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12420.11959326734198668No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC12310.11853406771174363No Hit
GTGCTGGTCATCTATTACGATAGCGACCGGCCCTCAGGGATCCCTGAGCG12240.11786003158340715No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT12180.11728228633054731No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG12130.11680083195316412No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG12030.11583792319839771No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG11990.11545275969649116No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11990.11545275969649116No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11690.11256403343219196No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC11580.11150483380194894No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC11390.10967530716789278No Hit
TAGTAGTGGTTACCACAACTGGTTCGACCCCTGGGGCCAGGGAACTCTGG11300.10880868928860302No Hit
AAGTAGAAGTGTTCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGC11280.10861610753764973No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC11240.10823094403574318No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11200.10784578053383662No Hit
TATAAAAAGTGCTCGTCGAATCGTCCGCGGTGATCGTGACTCTGCCCCGG11170.10755690790740668No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT11130.10717174440550013No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC11080.10669029002811693No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT11020.1061125447752571No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC10630.10235720063166814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15950.080.068691
GGTATCA7450.068.762591
ATCAACG20600.061.6089943
TCAACGC21500.059.0300184
CAACGCA22100.057.427395
TATCAAC22250.057.3643342
AACGCAG23250.054.8124436
CAGCGCT6700.047.2695279
CGCAGAG27550.045.9826978
GTACATG28600.039.860361
ACGCAGA32450.039.039247
TACATGG28700.038.6934552
GCAGCGC8500.038.106328
CATGGGA16950.037.8633464
GGCAGCG8250.037.516127
ATAGACG21300.036.9016344
ACATGGG30100.036.654193
TAGACGG21700.036.5537225
TTTCGCG800.002110091535.98939
AATAGAC23300.034.3530853
Produced by FastQC (version 0.11.5)