FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038520
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAGATTCCAGGCTGCTCAGCGCCATATAAA45700.440049300928244No Hit
GTCTATTACTGTGCGAAAGGGCCCCCCTTCAAATATGATAGTAGTGGTTA38870.37428263297769904No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27350.2633555444286099No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT23670.2279205022532065No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22120.2129954165543273No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21770.20962523591264492No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21590.2078920001540654No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20040.1929669144551862No Hit
CCTATGTGGTGACTCAGACACCCTCAGTGTCAGTGGCCCCAGGCAAGACG19420.18699688017563457No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19360.18641913492277473No Hit
GCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCGCCGCCGAACACCAG18300.1762123021222509No Hit
CTTTTTATATGGCGCTGAGCAGCCTGGAATCTGAGGACACGGCCGTCTAT17670.17014597696722258No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17560.16908677733697955No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17340.16696837807649345No Hit
GTCTGGGGCTGAATTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA17110.16475368794053075No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT16870.1624427069290914No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16360.15753187227978277No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC16070.15473943689096023No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG16040.1544505642645303No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA15670.15088780187189463No Hit
GCCTCTGTGACCTCCTATGTGGTGACTCAGACACCCTCAGTGTCAGTGGC15410.148384239109502No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG15380.14809536648307206No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT15140.14578438547163272No Hit
GACCTAGGACGGCCAGCTTGGTCCCGCCGCCGAACACCAGATGATCACCA15050.14491776759234296No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA15050.14491776759234296No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14790.14241420482995032No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14620.14077725994684742No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14330.13798482455802488No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14160.136347879674922No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14100.13577013442206215No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA14040.13519238916920231No Hit
ATCCCACATCTGACAGTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGC13750.13239995378037978No Hit
CACCAGCACTATCCCACATCTGACAGTAATAGTCGGCCTCATCCCCGGCC13340.12845202788583754No Hit
GTGCTGGTGATCATCTGGTGTTCGGCGGCGGGACCAAGCTGGCCGTCCTA13330.1283557370103609No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA13210.1272002465046412No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA13090.12604475599892154No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG12780.12305973885914571No Hit
CCCCTGTGCTGGTCATCTATTACGATAGCGACCGGCCCTCAGGGATCCCT12720.12248199360628588No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG12670.12200053922890267No Hit
CCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGGTGACTCAGACAC12600.12132650310056618No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12390.11930439471555675No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12360.11901552208912684No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12140.11689712282864076No Hit
TGATAGTAGTGGTTACCACAACTGGTTCGACCCCTGGGGCCAGGGAACTC12090.11641566845125755No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG12000.1155490505719678No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11960.11516388707006125No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11940.11497130531910797No Hit
CCCTCAGTGTCAGTGGCCCCAGGCAAGACGGCCAGAATTACGTGTGGGGG11860.11420097831529484No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11680.11246774255671532No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT11650.1121788699302854No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC11420.10996417979432269No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT11050.10640141740168702No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG11010.10601625389978046No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10960.10553479952239725No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10940.10534221777144398No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10820.10418672726572431No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10790.10389785463929438No Hit
TATAAAAAGTGCTCGTCGAATCGTCCGCGGTGATCGTGACTCTGCCCCGG10770.1037052728883411No Hit
GTGCTGGTCATCTATTACGATAGCGACCGGCCCTCAGGGATCCCTGAGCG10620.1022609097561915No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA10570.10177945537880831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)