FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076414
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTTCAGATCTCAGGCTGTTCAGATGCATGTAGG38690.3594341953932223No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCCGTGAAGGTCTCCTGCA31560.2931957406722692No Hit
GTGTATTACTGTGCGACAGATGGGGACTACTACGGTGACACCAAGGGCTA27830.25854364584630074No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26790.24888193576077605No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24660.22909401029715332No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT22790.21172151235491177No Hit
TTCCAGGACAGAGTCACGATCACCGCGGACGAGTCCACGAATACAGCCTA22630.2102350954186772No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT22570.2096776890675892No Hit
GCCTACATGCATCTGAACAGCCTGAGATCTGAAGACACGGCCGTGTATTA22510.20912028271650127No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22230.20651905307809076No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21880.20326751603007764No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21610.20075918745018181No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20550.19091167524762775No Hit
GTCCTGGAACCTCTGAGCGTAGTTTGTTGCACCATAGAGAGGGATGACCC18750.17418948471498882No Hit
GTAGTAGCCCTTGGTGTCACCGTAGTAGTCCCCATCTGTCGCACAGTAAT18020.16740770744341862No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17350.16118333652293634No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG16560.15384415290027814No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16200.15049971479375035No Hit
CATGTAGGCTGTATTCGTGGACTCGTCCGCGGTGATCGTGACTCTGTCCT15940.14808428727236916No Hit
CCCCTGGACAAGGCCTTGAGTGGATGGGAGGGGTCATCCCTCTCTATGGT15590.14483275022435607No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA15300.1421386195274309No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG15110.14037349941565233No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG15040.13972319200604974No Hit
CATCTGAACAGCCTGAGATCTGAAGACACGGCCGTGTATTACTGTGCGAC14990.1392586867134764No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14900.13842257718684445No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14840.1378651708357565No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14350.13331301896853812No Hit
GTTCAGATGCATGTAGGCTGTATTCGTGGACTCGTCCGCGGTGATCGTGA14240.13229110732487687No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC14060.13061888827161297No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT13850.1286679660428051No Hit
GTATTACTGTGCGACAGATGGGGACTACTACGGTGACACCAAGGGCTACT13850.1286679660428051No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13850.1286679660428051No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13550.12588093428736527No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG13310.1236513088830134No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC12900.11984236548391232No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT11960.11110966598353422No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11920.11073806174947556No Hit
GTCTCCGGGCACCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCT11910.11064516069096092No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG11900.11055225963244625No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11790.10953034798878498No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC11770.10934454587175566No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG11690.10860133740363836No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11670.10841553528660906No Hit
TCTCTATGGTGCAACAAACTACGCTCAGAGGTTCCAGGACAGAGTCACGA11470.10655751411631584No Hit
GTATTTCTGTCAGTGGTATGGTATCTCGCTCACTTTCGGCGGAGGGACCA11200.10404918553642No Hit
GTCCCAGGTGAAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGT11040.10256276860018544No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT11000.10219116436612678No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC10780.10014734107880426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12450.067.5859761
ATCAACG13450.062.5405923
TCAACGC13800.060.433444
CAACGCA14650.056.924415
AACGCAG15350.054.3032076
TATCAAC15600.053.4678272
CGCAGAG17450.048.2766238
GGTATCA5200.045.640531
CAGCGCT6200.045.289669
GCAGAGT21500.039.180829
GGCAGCG7600.037.895957
GTACATG25100.035.2428861
ACGCAGA24200.034.5135047
GTACACG12850.034.1275335
TACATGG25100.034.090422
GCAGCGC8300.032.9649128
TATGTAC13600.032.251512
ACATGGG26850.031.8640583
ACACGGG13800.031.8271067
GTATGTA15500.031.5513021
Produced by FastQC (version 0.11.5)