FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034719
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28230.27282769524866174No Hit
GTATGACAGTGATCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAACTCA26180.25301555301487655No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTCGTACAGCCGGGGG24740.23909873115309566No Hit
AGTCTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACCATT22460.21706376320527604No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20780.20082747103319837No Hit
TAATACAATAGTCTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCA19260.1861374924013186No Hit
GTAGGAGACAGAGTCATCATCACTTGCCGGGCCAGTCAAAATATAAGACG17580.169901200229241No Hit
GTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGCCGGGCCAGTCAAA17560.16970791103671626No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17400.16816159749651838No Hit
GATCAGGACTGAGCAGCGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC16510.15956022842916773No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16370.1582072040814946No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGTTTCCTCGTCCTCACTAGTGAC16310.15762733650392038No Hit
CTGTCATACTGGTGGCAGAAATAAGTTGTAAAGTCATCAGGCTGCAGGCT15630.15105550395807943No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14980.14477360520102558No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14950.14448367141223847No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14890.14390380383466428No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGCTTTTCAGGTGTT14060.13588230234488782No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14000.13530243476731363No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA13720.13259638607196736No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG13500.1304702049541953No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13380.12931046979904687No Hit
GTCATACTGGTGGCAGAAATAAGTTGTAAAGTCATCAGGCTGCAGGCTGC13140.1269909994887501No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA12670.12244870346441883No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12620.121965480483107No Hit
ATACAATAGTCTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGA12310.11896949799897363No Hit
GTATATTTCTGTGCGAGGGTTTCCTGGAGCAGAGCCTTCGGCAATACCTG12180.11771311824756286No Hit
GAAATATACGGCCGAGTCTTCGGCTCTCAGGCTGTCCATCTGCAGATACA12180.11771311824756286No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12090.11684331688120156No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12070.11665002768867683No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11800.11404062358959291No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11740.11346075601201872No Hit
GTATCTGCAGATGGACAGCCTGAGAGCCGAAGACTCGGCCGTATATTTCT11660.11268759924191979No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11620.11230102085687031No Hit
TTCCAGGAACACGCTGTATCTGCAGATGGACAGCCTGAGAGCCGAAGACT11590.11201108706808323No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11590.11201108706808323No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA11400.11017483973909825No Hit
TCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAGACGAACTGTGG11250.10872517079516275No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG11150.10775872483253907No Hit
CTCCAGAGACCATTCCAGGAACACGCTGTATCTGCAGATGGACAGCCTGA10930.10563254371476699No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10820.10456945315588098No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10700.10340971800073258No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC10590.10234662744184654No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10400.10051038011286155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14500.059.0599481
ATCAACG14550.056.815323
TCAACGC14600.056.1392564
CAACGCA15350.053.869895
TATCAAC16400.052.157012
AACGCAG16150.051.6591456
GGTATCA7150.044.2854161
CGCAGAG20200.041.3017438
GCAGAGT22350.037.0068449
TACATGG27850.032.520332
GTACATG27950.031.669221
GAGTTTA5950.031.4463651
GTCTATA2354.6189598E-827.5606041
TCTACAC8350.024.9656073
CAGAGTA22250.024.5666169
GTCTAGG2101.6471331E-523.9879321
TACGACA1500.001568691223.9658784
AGTTACC24350.023.6294049
ACATGGG37950.023.2982853
TAGGACC3102.6419002E-823.1927874
Produced by FastQC (version 0.11.5)