FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786658
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGGTCTCAGGCTATTCATTTGCAGAGAGA21930.2787742576824999No Hit
GTATATTACTGTGCGAGGGAGCGTGACTTCGGAATGGACGTCTGGGGCCA18670.23733312316152635No Hit
ACCACATACTACTCAGATTCTGTGAAGGGCCGATTCACCATCTCCAGAGA17990.22868896013261164No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14280.18152742360720925No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11640.14796773184789325No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11420.14517109086795024No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11100.1411032494425786No Hit
GCCGTATATTACTGTGCGAGGGAGCGTGACTTCGGAATGGACGTCTGGGG10760.13678116792812126No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10750.13665404788357838No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10360.1316963661464067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10100.12839124498829224No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10100.12839124498829224No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10000.1271200445428636No Hit
GTATGTGGTAGGACCTCTAGGACTAATGTACGAAACCCACTCCAGCCCCC9520.12101828240480615No Hit
ATATACGGCCGTGTCCTCGGGTCTCAGGCTATTCATTTGCAGAGAGAGGG9430.11987420200392038No Hit
TTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGTCTGGGGGAGACT9290.11809452138032028No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8970.11402667995494865No Hit
GTGCTGGGTTTTCCTTGTTGCTCTTTTAGAAGGTGTCCAGTGTGAGGTGC8850.11250123942043429No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8510.10817915790597693No Hit
GTATTACTGTCAGCAGTATGGTTTCTTACCTCCGTATACTTTTGGCCAGG8240.10474691670331962No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7930.10080619532249085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15700.076.28691
GGTATCA6050.075.13231
ATCAACG17950.065.426893
TCAACGC17950.065.02554
CAACGCA19200.060.78825
AACGCAG19850.058.734126
TATCAAC20050.058.57422
CGCAGAG22750.050.9307948
TAGACCG450.00988093648.0301785
ACGCAGA25400.045.9004867
ATACGGC10850.045.7673036
GCAGAGT26200.043.674899
TACGGCC11400.042.927947
GTAATAT12600.041.2290651
ATATACG12900.040.77244
AATATAC13200.039.2999233
GATTGCG850.00284253833.8670237
TATACGG15100.033.398475
TAATATA15150.033.2903632
GTGTAGA3700.031.2003751
Produced by FastQC (version 0.11.5)