FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980593
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT36130.36845051922663125No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30030.30624326300514076No Hit
GTCTACAAGATTACAGTTACCCTCGGACGTTCGGCCAAGGGACCAAGGTG19990.20385623801108102No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19910.2030404051426025No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19570.1995731154515686No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17930.18284854164775804No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATATA17830.18182875056215986No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17600.17948323106528397No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17040.17377240098593402No Hit
ACTTATTACTGTCTACAAGATTACAGTTACCCTCGGACGTTCGGCCAAGG16870.17203875614041705No Hit
CTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATG16200.1652061558669091No Hit
GTCTATTACTGTGCGAGAGATAGCAATTACTATGATGGTCGTGCTTATTA16060.16377844834707161No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15490.15796563915916184No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14890.1518468926455726No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14110.14389252217790666No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT14000.1427707519837486No Hit
AGATTACAGTTACCCTCGGACGTTCGGCCAAGGGACCAAGGTGGATGTCA13830.14103710713823167No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGA13820.14093512802967184No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA13500.1376717965557576No Hit
ATAGGAGACACAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAG12450.12696399015697646No Hit
GATCTATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCG12390.12635211550561753No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12230.1247204497686604No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12080.12319076314026309No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12060.12298680492314346No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11390.11615420464963547No Hit
GTGCAGCCACAGTTCGATTGACATCCACCTTGGTCCCTTGGCCGAACGTC11270.11493045534691763No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11190.11411462247843908No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC11180.11401264336987924No Hit
CTGTAATCTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT10870.11085129100452482No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10300.10503848181661504No Hit
GATTTAGGCTGGTATCAGCTGAAACCAGGAAAAGCCCCTAAGTTCCTGAT10290.10493650270805523No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACACAGTCACCATCACTT10270.10473254449093558No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA10120.10320285786253827No Hit
CTATAGGAGACACAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGA10040.10238702499405972No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG9940.10136723390846152No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9880.10075535925710259No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)