FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980593
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30880.3149114872327255No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23080.23536778255606555No Hit
GTCTACAAGATTACAGTTACCCTCGGACGTTCGGCCAAGGGACCAAGGTG18180.18539801936175354No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17330.1767297951341688No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16250.16571605140970821No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15300.15602803609652527No Hit
CTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATG14560.14848158206309856No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGA14070.14348460574366734No Hit
GTCTATTACTGTGCGAGAGATAGCAATTACTATGATGGTCGTGCTTATTA13640.13909950407559507No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13560.13828367120711652No Hit
ACTTATTACTGTCTACAAGATTACAGTTACCCTCGGACGTTCGGCCAAGG13240.13502033973320227No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATATA13200.13461242329896297No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12730.12981940519665142No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12680.1293095096538523No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11890.12125316007762649No Hit
AGATTACAGTTACCCTCGGACGTTCGGCCAAGGGACCAAGGTGGATGTCA11340.11564430910683639No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA11150.11370670604419979No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11140.11360472693563997No Hit
GATCTATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCG10860.11074931189596499No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10230.10432462805669629No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10020.10218306677694008No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9990.10187712945126061No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9950.10146921301702133No Hit
CTGTAATCTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT9870.10065338014854278No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG8850.037.4144829
CTATTAC10400.035.298973
GTCTATT10050.033.7045671
TCAACGC19800.032.3556374
TACACGG4250.032.180286
ACAGCCG9100.031.6405288
ATCAACG20150.031.43643
TCTATTA11000.031.4104182
GTACACG4500.030.3924855
AGTACCG950.004895906430.3082965
TATCAAC21500.030.1320862
CAGCCGT9350.029.2547999
CAACGCA22400.028.9214235
CCGTGCA4600.028.1669489
GACAGCC11300.028.028477
AATCCGC1804.9693426E-627.9930786
CGCAGAG23000.027.2280528
CATTAGC7700.027.1101828
AACGCAG24000.026.9933266
ACACGGG5200.026.3011917
Produced by FastQC (version 0.11.5)