FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366104
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT48080.3519497783477685No Hit
ACATACAACAGGGCCACTGGCGTCCCTGCCAGGTTCAGTGGCAGTGGGTC46690.3417748575511088No Hit
GTAATACACGGCCGTGTCTTCGGCTCTCAGGCTGGTCATCTGAAGATACA37420.27391765195036394No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36140.26454794071315213No Hit
CTACTACTGTCAGCAGGGGAAGAGTTACACTTTTGGCCAGGGGACCAAGG32820.24024525219163403No Hit
GTTGTATGTATCAACGATGAGGAGGCTGGGAGCCCGGCCAGGTTTCTGTT31520.23072913921634075No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC30070.22011501320543678No Hit
CTCTTATTTATCGCGATGGTTCCACGAAGTACGCGGACTCCGTGAAGGGC29890.21879739756270386No Hit
CTCCAGAGACAATGCCGAAAACACGCTGTATCTTCAGATGACCAGCCTGA28340.2074512628613927No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27330.20005797508828022No Hit
AAATAAGAGAGACCCATTGCAGCCCCTTCCCTGGAGCCTGGCGGACCCAG26730.19566592294583718No Hit
GTCTCCAGCCATTCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT24160.17685329960237287No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23440.17158283703144123No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22030.1612615144967001No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21080.15430743193783197No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG21010.15379502585454696No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT21010.15379502585454696No Hit
TTATTAGCCTGGTACCAACAGAAACCTGGCCGGGCTCCCAGCCTCCTCAT20830.15247741021181405No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20740.1518186023904476No Hit
GTGTATTACTGTGCGACTTATTCTCGACAAAATTGTCGTACTGCCAACTG20580.15064738848579612No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGCCTAGAGCCTGAAGATT20560.15050098674771467No Hit
CAGTAGTAGAGCGCAAAATCTTCAGGCTCTAGGCTGTTGATGGTGAGAGT20220.14801215720033029No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCAGGGACGCCAGTGGCCCTGTT19430.1422292885461136No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA19290.1412044763795436No Hit
CAACAGGGCCACTGGCGTCCCTGCCAGGTTCAGTGGCAGTGGGTCTGGGA18420.13483600077300117No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18280.13381118860643115No Hit
GCCTAGAGCCTGAAGATTTTGCGCTCTACTACTGTCAGCAGGGGAAGAGT17120.12531988779770795No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT16620.12165984434567208No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT16380.11990302348869486No Hit
GTTATTAGCCTGGTACCAACAGAAACCTGGCCGGGCTCCCAGCCTCCTCA15620.11433975744160035No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA15540.1137541504892746No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCTTGTTATTAGCCTGGTACCAAC15490.11338814614407103No Hit
TCTCTGGAGAGGGTGAATCGGCCCTTCACGGAGTCCGCGTACTTCGTGGA15440.11302214179886744No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15390.11265613745366386No Hit
GTAGTAGAGCGCAAAATCTTCAGGCTCTAGGCTGTTGATGGTGAGAGTGA15180.1111189192038088No Hit
GACTTCACTCTCACCATCAACAGCCTAGAGCCTGAAGATTTTGCGCTCTA15040.11009410703723874No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14940.10936209834683157No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGAGGAAACT14840.1086300896564244No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA14590.10680006793040647No Hit
CTCTACTACTGTCAGCAGGGGAAGAGTTACACTTTTGGCCAGGGGACCAA14550.10650726445424359No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC14530.10636086271616216No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC14030.1027008192641263No Hit
CACCATCAACAGCCTAGAGCCTGAAGATTTTGCGCTCTACTACTGTCAGC13800.10101719927618981No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13780.10087079753810838No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)