FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366104
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT44610.32654907679063966No Hit
ACATACAACAGGGCCACTGGCGTCCCTGCCAGGTTCAGTGGCAGTGGGTC44210.323621042029011No Hit
GTAATACACGGCCGTGTCTTCGGCTCTCAGGCTGGTCATCTGAAGATACA35140.2572278538090804No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33030.2417824704414891No Hit
GTTGTATGTATCAACGATGAGGAGGCTGGGAGCCCGGCCAGGTTTCTGTT29630.2168941749676452No Hit
CTACTACTGTCAGCAGGGGAAGAGTTACACTTTTGGCCAGGGGACCAAGG29600.21667457236052307No Hit
CTCTTATTTATCGCGATGGTTCCACGAAGTACGCGGACTCCGTGAAGGGC28890.21147731065863212No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC27720.2029128089808682No Hit
CTCCAGAGACAATGCCGAAAACACGCTGTATCTTCAGATGACCAGCCTGA27360.20027757769540241No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26590.19464111077926718No Hit
GTCTCCAGCCATTCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT24140.17670689786429145No Hit
AAATAAGAGAGACCCATTGCAGCCCCTTCCCTGGAGCCTGGCGGACCCAG23730.17370566223362205No Hit
GTGTATTACTGTGCGACTTATTCTCGACAAAATTGTCGTACTGCCAACTG22130.16199352318710727No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21580.15796747538986783No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21540.15767467191370496No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGCCTAGAGCCTGAAGATT20590.15072058935483684No Hit
TTATTAGCCTGGTACCAACAGAAACCTGGCCGGGCTCCCAGCCTCCTCAT20140.14742655024800455No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19820.14508412243870158No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCAGGGACGCCAGTGGCCCTGTT19800.14493772070062017No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT19680.14405931027213156No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19530.1429612972365208No Hit
CAGTAGTAGAGCGCAAAATCTTCAGGCTCTAGGCTGTTGATGGTGAGAGT19360.1417168824628286No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA18880.1382032407488742No Hit
CAACAGGGCCACTGGCGTCCCTGCCAGGTTCAGTGGCAGTGGGTCTGGGA18020.1319079660113725No Hit
GCCTAGAGCCTGAAGATTTTGCGCTCTACTACTGTCAGCAGGGGAAGAGT17730.12978514080919168No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17690.1294923373330288No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT17210.1259786956190744No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT17030.12466107997634147No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA16010.11719459133418832No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15810.11573057395337398No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA15590.1141201548344782No Hit
GTAGTAGAGCGCAAAATCTTCAGGCTCTAGGCTGTTGATGGTGAGAGTGA15480.11331494527503032No Hit
TCTCTGGAGAGGGTGAATCGGCCCTTCACGGAGTCCGCGTACTTCGTGGA15410.1128025391917453No Hit
GTTATTAGCCTGGTACCAACAGAAACCTGGCCGGGCTCCCAGCCTCCTCA14970.10958170095395374No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCTTGTTATTAGCCTGGTACCAAC14850.10870329052546511No Hit
GACTTCACTCTCACCATCAACAGCCTAGAGCCTGAAGATTTTGCGCTCTA14400.10540925141863285No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA13960.10218841318084128No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGAGGAAACT13890.10167600709755627No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC13890.10167600709755627No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC13880.10160280622851556No Hit
CTCTACTACTGTCAGCAGGGGAAGAGTTACACTTTTGGCCAGGGGACCAA13870.10152960535947483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19300.059.4013564
CAACGCA21000.054.2493255
AACGCAG22000.052.042436
ACGCAGA22400.051.436457
CGCAGAG23150.049.4589848
GGTATCA7550.047.773481
GTACACG9600.042.0603945
GCAGAGT28000.040.891989
TATAACG907.7280114E-540.0589872
ATACACG18400.039.9704324
TATGTAC11250.037.174742
GTAATAC19650.037.0785371
ACACGGG11050.036.4942787
TACATGG33450.034.2746852
ACGGGGT12000.034.2052429
GTACATG33800.033.721291
ACATGGG34000.032.6586573
AATACAC23150.032.0806243
TAGGACG900.00372744232.0460134
AAGCGCT3250.031.0599824
Produced by FastQC (version 0.11.5)