FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1961811
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTTATAGTACAGGCAACAAACACCCCTGGACCCCTGCCCGGTTCTCAG57750.294370864471654No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC45550.2321834264360838No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT41910.21362914164514318No Hit
CCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACC40480.2063399583344165No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC39090.19925466826315072No Hit
CTATAAATCAGTGCCCTGGGTGGTTGTCCAGGTTTCTGCTGGAACCAGTT36600.18656231410671056No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCACCA35290.17988481051436658No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34300.17483845283770963No Hit
CTACTATGGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC34240.17453261297851833No Hit
CCCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG32330.164796710794261No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA31800.16209512537140428No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30950.15776239403286044No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA30430.15511178191986894No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT29180.14874011818671626No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCA26260.13385591170607158No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC26260.13385591170607158No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26240.13375396508634113No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAG26120.13314228536795847No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAG25900.1320208725509236No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA24760.12620991522628836No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG23990.12228497036666632No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23800.1213164774792271No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23150.1180032123379877No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA22450.1144350806474222No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21980.11203933508375678No Hit
TCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCAC21600.11010234930887838No Hit
GTCCAGGGGTGTTTGTTGCCTGTACTATAAATCAGTGCCCTGGGTGGTTG21490.10954164290036095No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA21260.10836925677346085No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20940.10673811085777375No Hit
TAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGA20750.10576961797033456No Hit
GTACATGGGAGGCGCCAGGGGCCCCTTCCAATATCAGCACCATGGCCTGG20500.10449528522370401No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20440.10418944536451269No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCACCAT20150.10271121937842126No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG20120.1025582994488256No Hit
GTATTACTGCCTGCTCTACTATGGTGGTGCTTGGGTGTTCGGCGGAGGGA19800.10092715353313851No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19670.10026450050489064No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)