FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215098
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA51420.42317574384946727No Hit
GTCTATGGTGGGGCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCC42110.3465564094418722No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG37480.3084524869598995No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG36530.3006341875305531No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29250.24072132453514036No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG28850.2374294089859419No Hit
GTATAATGACTGGCCTCGCATGCACACTTTTGGCCAGGGGACCAAGCTGG28220.23224464199595427No Hit
GTGTATTACTGTGCGAGAGCCGCATATTGTGGTGGTGATTGCAACTCCTA28110.23133936521992465No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT26790.22047604390756959No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT26630.2191592776878902No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG22880.18829756941415424No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC21430.1763643755483097No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20930.17224948111181157No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT18770.15447313714613964No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18160.14945296593361193No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC17310.14245764539156514No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16770.13801355940014715No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGTGAAAGAGCCACCCTCTCCT16570.1363676016255479No Hit
TCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT16340.13447475018475877No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGT16010.13175891985667001No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGGGAGT15560.1280555148638217No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15540.12789091908636177No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC15270.12566887609065275No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT14380.1183443639936861No Hit
ATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGAGAACT14100.11604002310924716No Hit
GTCCCAGACCCACTGCCACTGAACCGGGCTGGGATACCAGTGGCCCTGGT13950.11480555477829772No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC13890.11431176744591794No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13720.11291270333750858No Hit
CAGTAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGA13460.11077295823052956No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC13210.1087155110122805No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13110.10789253212498087No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC12870.10591738279546177No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT12750.10492980813070223No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12680.10435372290959247No Hit
CGTCCATACCGTGGTAGTGGTAGTAGGAGTTGCAATCACCACCACAATAT12460.10254316935753331No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG12260.10089721158293406No Hit
GACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCA12170.10015653058436438No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)