FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215098
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA42460.34943683554742083No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG38940.3204679787144741No Hit
GTCTATGGTGGGGCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCC37630.309686955290849No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG30930.25454736984177406No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG26420.217431022024561No Hit
GTATAATGACTGGCCTCGCATGCACACTTTTGGCCAGGGGACCAAGCTGG24990.20566242393617634No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24730.2035226788291973No Hit
GTGTATTACTGTGCGAGAGCCGCATATTGTGGTGGTGATTGCAACTCCTA24690.20319348727427747No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT23990.19743263506318007No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT21080.173483949442761No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC19640.16163305346564638No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18620.15323866881519022No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15670.12896079163985127No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG15640.1287138979736614No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14940.122953045762564No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14920.12278844998510408No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC14740.12130708798796476No Hit
TCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT14270.11743908721765652No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC13820.1137356822248082No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT13770.11332419278115839No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGTGAAAGAGCCACCCTCTCCT13540.11143134134036926No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGT13200.10863321312355052No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT13200.10863321312355052No Hit
GTCCCAGACCCACTGCCACTGAACCGGGCTGGGATACCAGTGGCCCTGGT12820.10550589335181196No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGGGAGT12710.10460061657578236No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12440.10237857358007338No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15500.061.766484
ATCAACG15550.061.5678833
CAACGCA17000.055.893075
TATCAAC17650.054.2425232
AACGCAG18300.051.1358226
ACGCAGA19900.047.0243957
GTATCAA21500.046.9231831
GTCATAG17450.045.8379751
CGCAGAG20900.044.4300088
ATAGGTG17450.043.7263684
TCATAGG18350.042.758592
CATAGGT18500.041.6336983
TAGGTGG19500.039.4986345
GTGGTCA20500.036.518468
GCAGAGT25250.035.920489
GGTATCA11200.035.3868981
CAGAGCG15550.035.1816449
AGGTGGT23150.033.8928766
GTATAGG4100.031.6363581
CCCGTTG6250.029.9451358
Produced by FastQC (version 0.11.5)