FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897238
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34110.3801666893288069No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30800.34327569719517004No Hit
GTAATAAACCCCAACATCCTCAACCTCCACTCTGCTGATTTTCAGTGTAA22050.24575419231017856No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21080.23494323691149954No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20960.23360579913021964No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18520.206411230910862No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17100.1905848838323834No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16360.1823373508478241No Hit
GTGATGGAAACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT16200.18055410047278425No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16190.18044264732434429No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15500.17275238008198493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15450.172195114339785No Hit
TTATTACTGCTTGCAAGTTCTCCAACTTCCGTACACTTTTGGCCAGGGGA15220.1696316919256652No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13640.15202209447214673No Hit
GTATCAACGCAGAGTACATGGGGATCAGGACTCCTCAGTTCACCTTCTCA13270.1478983279798671No Hit
GTTTATTACTGCTTGCAAGTTCTCCAACTTCCGTACACTTTTGGCCAGGG13220.14734106223766716No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12950.14433182722978743No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG12750.1421027642609876No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGTTGAGGATGTTGGGGTTTA12190.13586138794834815No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGTTGTG12040.1341895907217483No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT11830.13184907460450848No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11790.13140326201074853No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11770.13118035571386855No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11630.1296200116357087No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA11510.12828257385442882No Hit
TTCCATCACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC11460.12772530811222887No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG11400.1270565892215889No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC11350.12649932347938897No Hit
CAGTAATAAACCCCAACATCCTCAACCTCCACTCTGCTGATTTTCAGTGT11310.126053510885629No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11270.12560769829186905No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11220.1250504325496691No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11050.12315572902618925No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA10970.12226410383866934No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGT10860.12103811920582944No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA10770.12003504086986953No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10620.11836324364326968No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT10610.1182517904948297No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10510.11713725901042979No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAACTCAACTTCAGGGAGA10440.11635708697134986No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC10380.11568836808070991No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10290.11468528974475001No Hit
GGCCAAAAGTGTACGGAAGTTGGAGAACTTGCAAGCAGTAATAAACCCCA10290.11468528974475001No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAG10160.11323639881503013No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGT10080.11234477362751019No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG9980.11123024214311031No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9930.11067297640091035No Hit
GTACATGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCC9790.10911263232275048No Hit
GAGTGGAGGTTGAGGATGTTGGGGTTTATTACTGCTTGCAAGTTCTCCAA9780.1090011791743105No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9700.10810955398679058No Hit
CTCCTGCATAGTGATGGAAACAACCATTTGGATTGGTACCTGCAGAAGCC9640.10744083509615064No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT9350.10420869379139092No Hit
CCTGCATAGTGATGGAAACAACCATTTGGATTGGTACCTGCAGAAGCCAG9320.10387433434607095No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTGATGGAAACAACCATTTGGATTGG9280.10342852175231097No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22300.075.2967151
ATCAACG23950.069.8005753
TCAACGC24200.069.377254
GGTATCA6900.065.798571
CAACGCA25550.065.7115255
AACGCAG25900.065.379966
TATCAAC25850.064.6701662
ACGCAGA28850.058.675027
CGCAGAG29450.057.450788
TATAACG657.5609813E-444.3428043
GTATAGG2104.0381565E-1034.316711
GTGCTAG4300.033.5186461
GCAGAGT53850.033.424719
ATACACG5200.033.2571034
GTACATG24400.033.079061
TACATGG24100.031.992142
TACACGG5250.031.5678545
ACATGGG24800.031.0891343
CAGAGTA34500.029.4247889
TAACGCT1000.00625871828.8228235
Produced by FastQC (version 0.11.5)