FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083373
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATATACTTTGGTTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG27440.25328303363661453No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC24530.22642247868462662No Hit
CTCCTGCACTGGGACCAGCAGTGACGTTGGTTATTATAACTTTGTCGCCT21580.19919270648243956No Hit
GACCTAGGACGGTCACCGTGGTCCCTCCGCCGAATAGCACAATACTGCCG21100.1947620994800498No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT18440.17020915234180656No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA18210.16808615315316147No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGGACCAGCAGTGACGTT17610.16254789440017428No Hit
GTCTGGGGCTGAGGTGAAGGAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA16540.1526713329573471No Hit
TCTATAGGAAGCGCAGTAATATTCAGCCTCGTCCTCAGCCTGGAGCCCAG16130.14888685614280586No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC15060.1390102946999787No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15000.13845646882467996No Hit
GCCTACATGGAGTTGAGCAGGCTGACATCTGACGACACGGCCGCATATTT14740.1360565566983855No Hit
GCCTTGGGCTGACCTAGGACGGTCACCGTGGTCCCTCCGCCGAATAGCAC14690.1355950351356366No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT14570.13448738338503913No Hit
CCCTAACACTGGTGGCAGAAACTTTCCACAAAAGTTTCAGGGCCGGGTCA14010.12931834188225108No Hit
TCCTATAGAAGCGGCAGTATTGTGCTATTCGGCGGAGGGACCACGGTGAC13960.12885682031950216No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT13530.12488773487986132No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13400.12368777881671408No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT12920.11925717181432434No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTTCTCAGCCTCC12680.11704186831312945No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12460.11501117343703415No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG11800.10891908880874823No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG11750.10845756724599931No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG11560.10670378530755334No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11200.10338083005576103No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11140.10282700418046231No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11020.10171935242986488No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)