FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2456053
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAACACCGTACACCTTTGGCCAGGGGACCCAGCTGCAGATCAAACGA121590.49506260654798573No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT96670.39359899806722415No Hit
GTGTTGAACCATATTGTTGACAAAAATACACTGCAAAATCTTCAGGCTCC95350.38822452121350803No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC69270.28203788761887466No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCATCCTCTCCT65880.26823525388092195No Hit
ATATTATACTGGGAACACCGACTTTAATCCGTCCTTCGAGAGTCGAGTCT53250.2168112821669565No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT52910.2154269472197872No Hit
GTTAGGGCCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC50890.20720236900425193No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG42670.1737340358697471No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42500.17304186839616245No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG40370.16436941710948422No Hit
CCTTTGGCCAGGGGACCCAGCTGCAGATCAAACGAACTGTGGCTGCACCA40120.16335152376597736No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39680.16156003148140533No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT39440.16058285387163876No Hit
GTATAATATATCTCCCCAAGCCACTCCAGGCCCTTCCCTGGGGACTGGCG38510.15679629063379333No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCATCCTCTCCTGC36500.14861242815199835No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35700.14535516945277646No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCATCCTCTCCTGCACGGCCAGGCAGA34330.139777113930359No Hit
CTCCTGCACGGCCAGGCAGAGTCTTGTCAGTCGTTTCTTAGCCTGGTACC32440.1320818402534473No Hit
CCGTACACCTTTGGCCAGGGGACCCAGCTGCAGATCAAACGAACTGTGGC32060.1305346423713169No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31510.12829527701560187No Hit
GGCCAAAGGTGTACGGTGTTGAACCATATTGTTGACAAAAATACACTGCA31460.1280916983469005No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30820.12548589138752297No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA30620.12467157671271752No Hit
TCTATGGTGCATCCACTAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGC30530.12430513510905505No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG29330.11941924706022222No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA29060.11831992224923485No Hit
CATCTATGGTGCATCCACTAGGGCCCCTGGCATCCCAGACAGGTTCAGTG28170.11469622194635051No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC27670.11266043525933683No Hit
CACTAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGAGA27670.11266043525933683No Hit
GTATTTTTGTCAACAATATGGTTCAACACCGTACACCTTTGGCCAGGGGA27650.11257900379185629No Hit
GTCTTAATGTGGGGTACTTCGATCTCTGGGGCCGTGGCGCCCCGGTGACT27220.11082822724102451No Hit
GCTCAGTTAGGGCCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT26880.10944389229385523No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26720.10879244055401086No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA25950.10565732905600977No Hit
CATTAAGACTATCAATGTCATCGTCTCGCCTGGCACAATAATAAACGGCC25580.10415084690761968No Hit
GTGCAGCCACAGTTCGTTTGATCTGCAGCTGGGTCCCCTGGCCAAAGGTG24900.10138217701328106No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG24860.10121931407831998No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC24740.10073072527343668No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24590.10011998926733258No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)